HDAC2 siRNA转染人胰腺癌细胞对Bax和Bcl-2基因表达的影响

Effects of siRNA-mediated HDAC2 on the Expression of Apoptosis-related Genes of Bax and Bcl-2 in Human Pancreatic Cancer Cells PaTu8988

目的:探讨组蛋白去乙酰化酶2(HDAC2)小干扰RNA(small interference RNA,siRNA)转染人胰腺癌细胞PaTu8988对凋亡相关基因Bax和Bcl-2表达的影响。方法:培养人胰腺癌细胞PaTu8988,将其随机分为三组:空白对照组、Negative SiRNA组、HDAC2SiRNA组,合成针对HDAC2基因的siRNA,利用阳离子脂质体(Lipofectamine2000)将HDAC2siRNA瞬时转染人胰腺癌细胞PaTu8988,应用免疫印迹法(Western blot)检测Bax和Bcl-2的表达。结果:与对照组和Negative SiRNA组对比,HDAC2siRNA组的Bax蛋白表达增加(P<0.01),Bcl-2蛋白的表达降低(P<0.01)。结论:采用siRNA干扰沉默人胰腺癌细胞HDAC2基因,可增加Bax的表达,抑制Bcl-2的表达。

Objective：To observe the effects of siRNA-mediated Histone Deacetylases 2（HDAC2）on the expression of apoptosis-related genes of Bax and Bcl-2in human pancreatic carncer cells PaTu8988.Methods：Human pancreatic cancer cell line PaTu8988 cells were cultured and divided into three groups：control group,negative siRNA group,HDAC2 siRNA group.Transient transfection of siRNA against HDAC2 on Human pancreatic cancer cells PaTu8988 were adopted by Lipofectamine 2000.Expression of Bax and Bcl-2genes were detected Western blot.Results：Compared with control group and negative siRNA group,the protein expression of Bax in HDAC2 siRNA group was obviously higher（P〈0.01）,but the protein expression of Bcl-2in HDAC2 siRNA group was deeply decreased（P〈0.01）.Conclusion：Transfection of HDAC2 siRNA in Human pancreatic cancer cells PaTu8988 can increase the expression of Bax gene,inhibit the expression of Bcl-2gene.