丹参饮精品中药与普通中药四种酚性成分的比较研究

Determination of Four Phenolic Constituents between Boutique Salvia Decoction and Common Salvia Decoction by High Performance Liquid Chromatograph

目的:建立高效液相色谱法测定丹参饮中丹参素、原儿茶酸、原儿茶醛、丹酚酸B的含量,比较丹参饮精品中药与普通中药上述四种成分的含量变化。方法:采用Genmini 5μm C18110A(250 mm×4.60 mm,5μm)色谱柱,以乙腈(A)-0.05%三氟乙酸(B)溶液为流动相,梯度洗脱,流速0.8 m L/min,柱温为25℃,检测波长288 nm,进样量15μL。结果:在40 min内,丹参饮中丹参素、原儿茶酸、原儿茶醛、丹酚酸B四种酚性成分能够实现完全分离,并且浓度与峰面积在一定范围内线性关系良好,相关系数r≤0.999 8。与普通中药相比,丹参饮精品中药中丹参素、丹酚酸B、原儿茶醛和原儿茶酸含量明显增加。结论:该分析方法专属性好,灵敏度高,定量准确,经方法学验证可用于测定丹参饮中四种酚性成分的含量。

Objective： To establish an high performance liquid charomatography（HPLC） method for simultaneous deter- mination of Danshensu, protocatechuic acid, protocatechualdehyde and salvianolic acid B in Salvia mihiorrhiza decoction, and to study the discrepancy of four phenolic constituents between Boutique salvia decoction and common salvia decoction. Methods：Analysis was performed on a Waters Symmetry ClS （4.60 mm × 250 mm,5 μm）column with gradient e- lution of acetonitrile （A） and 0.05 % trifluoroacetie acid （B） at the flow rate of 0.8 ml/min. Detection wavelength was 288 nm and column temperature was set at 25℃. Results： Danshensu, protocatechuic acid, protocatechualdehyde and salvianolic acid B had a good linearity in certain ranges, with correlation coefficient r≤0.999 8. Compared with the common salvia decoction, the contents of Danshensu, salviandic acid B, protocatechualdehyde and protocatechuic acid were increased obviously. Conclusion：This method is simple, accurate with high stability and good repeatability, which is help- ful to control the quality of salvia miltiorrhiza decoction.