常用松香酸检查方法的对比分析及在三种中成药中的应用

Comparative Analysis of Commonly used Abietic Acid Inspection Method and the Application of Three Kinds of Traditional Chinese Medicine

目的:通过对松香酸分析和比较,建立可同时检验乳香、没药、沉香、血竭中是否含有松香酸的统一方法,并应用三种中成药对该方法进行验证。方法:采用两种TLC法和三种HPLC法检测以上四味药材中松香酸。结果:TLC法可同时检测沉香、没药中松香酸。HPLC法以乙腈-四氢呋喃-0.1%甲酸(35∶25∶40)为流动相,对四味药材中松香酸可同时检测。结论:同时检测乳香、沉香、没药、血竭四味药材中松香酸,TLC法干扰较多,首选HPLC法。

Objective： To develop one method for abietic acid test in frankincense, myrrh, aloes and sanguis draconis by comparing and analyzing different detection methods of abietic acid. Apply three kinds of traditionnal Chinese medicine to validate the method. Methods： Two TLC methods and three HPLC methods were tested in frankincense, myrrh, aloes and sanguis draconis. Results： One TLC method of abietic acid test could be only used for both aloes and myrrh. But one HPLC method of abietic acid test could be used for frankincense, myrrh, aloes and sanguis draconis. The mobile phase was acetonitrile - tetrahydrofuran - water （ containing 0.1% formic acid） （35：25：40）. Conclusion ： HPLC method is suitable for abietic acid test in frankincense, myrrh, aloes and sanguis draconis.