稳消II方对腹主动脉粥样硬化模型兔脂质代谢的影响

Effect of Wenxiao Ⅱ Decoction on Lipid Metabolism in Experimental Atherosclerosis Rabbits

目的:观察稳消II方对动脉粥样硬化模型兔脂质代谢的影响,探讨其防治动脉粥样硬化的作用机制。方法:将38只健康新西兰大白兔随机分为空白组9只和造模组29只。空白组予标准饲料,造模组予高脂饲料喂养8周,第8周末随机处死2只。造模成功后随机分为稳消II方干预组(9只)、辛伐他汀对照组(9只)、模型对照组(9只),并改用标准饲料喂养。分别予相应药物灌胃干预8周[模型对照组与空白对照组均予生理盐水10 m L/(kg·d)]。分别于第0、8、16周末进行耳缘静脉采血,检测TC、TG、HDL-C、LDL-C、ALT、AST、Cr、BUN、UA、CK水平。结果:稳消II方干预组、辛伐他汀对照组TC、TG、LDL-C水平均低于模型对照组,HDL-C水平高于模型对照组,差异均有统计学意义(P<0.05)。稳消II方干预组、辛伐他汀对照组与空白对照组比较,TC、TG水平差异均无统计学意义(P>0.05);稳消II方干预组、辛伐他汀对照组LDL-C、HDL-C水平高于空白对照组,差异有统计学意义(P<0.05)。稳消II方干预组与辛伐他汀对照组比较,TC、TG、LDL-C、HDL-C水平差异均无统计学意义(P>0.05)。稳消II方组、辛伐他汀组干预前后自身对照比较,TC、LDL-C、TG水平均显著下降,HDL-C水平明显上升,差异均有统计学意义(P<0.05)。结论:稳消II方可降低动脉粥样硬化兔TC、TG、LDL-C,升高HDL-C水平,调节脂质代谢,改善和延缓动脉粥样硬化,且其对实验兔ALT、AST、Cr、BUN、UA、CK无不良影响。

Objective： To observe the effect of different doses of Wenxiao Ⅱ decoction on lipid metabolism in experimental atherosclerosis rabbits, and to explore its mechanism in treating atherosclerosis. Methods： The 38 New Zealand rabbits were randomly divided into two groups according to the weight： the blank control group with 9 rabbits and the model group with 29 rabbits, regardless of male or female. Except for those in the blank group, all rabbits were fed with a high-cholesterol diet. After 8 weeks, two rabbits randomly from the model group were killed to confirm whether the model is successful or not. Tben, the model group was randomly divided into three groups： Wenxiao Ⅱ decoction group, simvastatin group and model control group, fed with standard diet rather than the high-fat diet. Give the corresponding medication orally 8 weeks [blank con- trol group and model control group feed with 0.9% NS 10 mL（kg·d）]. Respectively, to test TC, TG, HDL-C, LDL-C, ALT, AST, Cr, BUN, UA, CK levels on the 0,8,16 weekend through the ear vein blood collection. Results： Compared with the model group, TC, TG and LDL-C levels were significantly lower while HDL-C levels increase a lot in Wenxiao Ⅱ decoction group and simvastatin group, of which has meaningful statistics significance （P〈0.05）. Compared with the blank control group, TC, TG levels of both Wenxiao Ⅱ decoction group and simvastatin group has no statistical difference （P〉0.05）. LDL-C, HDL-C levels decreased, and statistically significant （P〈0.05）. Compared with the simvastatin group, the TC, TG and LDL-C,HDL-C levels of Wenxiao Ⅱ decoction group were not significantly different （P〉0.05）. The levels of TC, LDL-C and TG decreased apparently,and the level of HDL-C increased significantly ＇after treatments both in Wenxiao Ⅱ decoetiuu group and simvastatin group, and there was statistical difference （P〈0.05）. Conelusion： Wenxiao Ⅱdecoction can reduce TC, TG, LDL-C, increased HDL-C levels, regulate lipid metabolism effeet, and prevent the occurrence of atheroselerosis.