水飞蓟宾对快速老化小鼠学习记忆影响

Effect of silibinin on learning and memory in mice with accelerated senescence

目的探讨水飞蓟宾对快速老化小鼠(SAMP8)学习记忆及氧化应激因子表达影响。方法以SAM P8小鼠为模型,采用新物体辨别、Y迷宫及M orris水迷宫进行学习记忆能力评价,酶联免疫实验检测氧化应激相关指标。结果与对照组比较,模型组小鼠优先指数(0.45±0.07)、自发交替反应率[(0.58±0.06)%]明显下降,逃避潜伏期[(50.62±11.24)s]明显延长(P<0.01),小鼠海马组织中谷胱甘肽(GSH)、超氧化物歧化酶(SOD)水平[分别为(21.13±6.17)mg/mL、(15.21±0.09)μg/mL]降低,丙二醛含量[(4.02±0.12)nmol/mL]升高(P<0.05);与模型组比较,水飞蓟宾200 mg/kg组小鼠优先指数(0.68±0.09)、自发交替反应率[(0.70±0.09)%]升高,逃避潜伏期[(23.45±11.94)s]明显缩短(P<0.01),小鼠海马组织中GSH、SOD水平[分别为(42.37±12.08)mg/mL、(32.24±0.24)μg/mL]升高,丙二醛含量[(2.54±0.21)nmol/mL]明显降低(P<0.05)。结论水飞蓟宾可改善快速老化小鼠学习记忆障碍,其机制可能与提高小鼠抗氧化应激能力有关。

Objective To investigate the effect of silibinin on learning memory and expression of oxidative stress factor in senescence-accelerated mouse prone 8 （SAMP8）.Methods Forty male SAMP8 mice of 13 weeks old were assigned into a model group （gavaged with normal saline）,low- and high-dose groups （with 100 and 200 mg/kg silibinin）,and positive control group （with 2.6 mg/kg memantine） and 10 senescence accelerated mouse resistant 1 （SAMR1） mice （with normal saline gavage） were included in a normal control group.All the mice were treated once a day for 6 weeks consecutively.We adopted novel object recognition,Y maze and Morris water maze test to evaluate the ability of learning and memory of the mice.Enzyme-linked immunosorbent assay was used to detect oxidative stress-related indicators.Results Compared with the control group,the model group showed significantly lower preference index （0.45±0.07）,spontaneous alternation rate （0.58%±0.06%） and prolonged escape latency （50.62±11.24 s）（P〈0.01 for all）;the model group also showed significantly decreased glutathione （GSH,21.13±6.17 mg/mL） and superoxide dismutase （SOD,15.21±0.09 μg/mL） and increased malondialdehyde （MDA,4.02±0.12 nmol/mL） in hippocampus tissue （P〈0.05 for all）.Compared with the model group,the high-dose silibinin group had significantly higher preference index （0.68±0.09）,spontaneous alternation rate （0.70%±0.09%） and shortened escape latency （23.45±11.94 s） （P〈0.01 for all）;in addition,significantly increased GSH （42.37±12.08 mg/mL）,SOD （32.24±0.24 μg/mL） and decreased MDA （2.54±0.21 nmol/mL） in hippocampus tissues were observed in the mice with high-dose silibinin treatment （P〈0.05 for all）.Conclusion Silibinin could improve learning and memory deficit in SAMP8 mice and the effects may be associated with the increased anti-oxidative stress capability of the mice.