摘要
目的探讨水飞蓟宾对快速老化小鼠(SAMP8)学习记忆及氧化应激因子表达影响。方法以SAM P8小鼠为模型,采用新物体辨别、Y迷宫及M orris水迷宫进行学习记忆能力评价,酶联免疫实验检测氧化应激相关指标。结果与对照组比较,模型组小鼠优先指数(0.45±0.07)、自发交替反应率[(0.58±0.06)%]明显下降,逃避潜伏期[(50.62±11.24)s]明显延长(P<0.01),小鼠海马组织中谷胱甘肽(GSH)、超氧化物歧化酶(SOD)水平[分别为(21.13±6.17)mg/mL、(15.21±0.09)μg/mL]降低,丙二醛含量[(4.02±0.12)nmol/mL]升高(P<0.05);与模型组比较,水飞蓟宾200 mg/kg组小鼠优先指数(0.68±0.09)、自发交替反应率[(0.70±0.09)%]升高,逃避潜伏期[(23.45±11.94)s]明显缩短(P<0.01),小鼠海马组织中GSH、SOD水平[分别为(42.37±12.08)mg/mL、(32.24±0.24)μg/mL]升高,丙二醛含量[(2.54±0.21)nmol/mL]明显降低(P<0.05)。结论水飞蓟宾可改善快速老化小鼠学习记忆障碍,其机制可能与提高小鼠抗氧化应激能力有关。
Objective To investigate the effect of silibinin on learning memory and expression of oxidative stress factor in senescence-accelerated mouse prone 8 (SAMP8).Methods Forty male SAMP8 mice of 13 weeks old were assigned into a model group (gavaged with normal saline),low- and high-dose groups (with 100 and 200 mg/kg silibinin),and positive control group (with 2.6 mg/kg memantine) and 10 senescence accelerated mouse resistant 1 (SAMR1) mice (with normal saline gavage) were included in a normal control group.All the mice were treated once a day for 6 weeks consecutively.We adopted novel object recognition,Y maze and Morris water maze test to evaluate the ability of learning and memory of the mice.Enzyme-linked immunosorbent assay was used to detect oxidative stress-related indicators.Results Compared with the control group,the model group showed significantly lower preference index (0.45±0.07),spontaneous alternation rate (0.58%±0.06%) and prolonged escape latency (50.62±11.24 s)(P〈0.01 for all);the model group also showed significantly decreased glutathione (GSH,21.13±6.17 mg/mL) and superoxide dismutase (SOD,15.21±0.09 μg/mL) and increased malondialdehyde (MDA,4.02±0.12 nmol/mL) in hippocampus tissue (P〈0.05 for all).Compared with the model group,the high-dose silibinin group had significantly higher preference index (0.68±0.09),spontaneous alternation rate (0.70%±0.09%) and shortened escape latency (23.45±11.94 s) (P〈0.01 for all);in addition,significantly increased GSH (42.37±12.08 mg/mL),SOD (32.24±0.24 μg/mL) and decreased MDA (2.54±0.21 nmol/mL) in hippocampus tissues were observed in the mice with high-dose silibinin treatment (P〈0.05 for all).Conclusion Silibinin could improve learning and memory deficit in SAMP8 mice and the effects may be associated with the increased anti-oxidative stress capability of the mice.
作者
孙一峰
甘世明
张晓东
任立震
唐迪莹
杜运
胡焱军
冯思琪
柴宁宁
夏梁慧
金戈
SUN Yi-feng GAN Shi-ming ZHANG Xiao-dong et al(School of Basic Medical Sciences, Shenyang Medical College, Shenyang , Liaoning Province 110034, China)
出处
《中国公共卫生》
CAS
CSCD
北大核心
2017年第3期465-467,共3页
Chinese Journal of Public Health
基金
辽宁省教育厅一般项目(L2014409)
沈阳市科技局项目(F13-220-9-27)
辽宁省大学生创新创业培训项目(1169)