荜茇酰胺对胶质瘤细胞增殖影响

Impact of piperlongumine on glioma proliferation

目的探讨荜茇酰胺(piperlongumine)对胶质瘤增殖影响及机制。方法采用荜茇酰胺处理2种不同人脑胶质瘤细胞系,同时设正常神经元和胶质细胞对照;采用噻唑蓝法检测细胞增殖力,采用试剂盒法检测细胞内活性氧(ROS)及过氧化物还原酶4(PRDX4)活性,采用Annexin V染色法检测细胞凋亡率,采用RT-PCR法检测PRDX1~6基因表达。结果与对照组比较,荜茇酰胺能够明显抑制胶质瘤细胞增殖能力,增强2种胶质瘤细胞内的ROS水平(P<0.05),但对正常神经元则无明显影响;与对照组比较,胶质瘤细胞凋亡率明显升高(P<0.05),胶质瘤细胞内PRDX4表达上升(P<0.05)。结论荜茇酰胺可通过提高PRDX4的表达从而介导胶质瘤细胞的凋亡。

Objective To investigate the effect of piperlongumine on the proliferation of glioma cells and molecular mechanism involved.Methods We treated two kinds of high-grade glioma （HGG） cells with piperlongumine and divided the cells of every kind into a control and a treatment group.Then we assessed cell growth level of each group using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay.We assessed reactive oxygen species （ROS） level and peroxiredeoxin 4 （PPDX4） activity of cells in each group using detecting kits.We assessed cell apoptosis level using annexin V staining method.We also examined the expression of PRDX1-6 using reverse transcriptase （RT-PCR）.Results Compared with the control group,piperlongumine treatment inhibited cell proliferation and increased ROS levels （both P〈0.05）,but had little effect on normal brain cells （P〈0.05）.Compared with the control group,piperlongumine treatment promoted cell apoptosis （P〈0.05） and downregulated the expression of PRDX4 （P〈0.05）.Conclusion Piperlongumine preferentially promotes HGG cell apoptosis by downregulating and inactivating PRDX4.