抑制EphA4受体活性对少突胶质前体细胞发育影响的研究

Effect of inhibit EphA4 receptor on oligodendrocyte progenitor cells

目的探讨抑制EphA4受体活性对少突胶质前体细胞(OPC)发育的影响。方法将分离纯化的OPC随机分为正常组、EphA4受体抑制剂处理组(EphA4-FC组)。EphA4-FC组加入250 nmol/L EphA4-FC,培养至第2天。免疫细胞化学染色法观察正常组OPC特异性标志物NG2与EphA4受体的共表达情况;通过细胞计数观察两组OPC数量变化情况;通过Western blot检测两组细胞蛋白样品中OPC特异性蛋白血小板源性生长因子α受体(PDGFαR)的表达变化情况。结果免疫细胞化学染色显示,正常体外培养OPC高表达EphA4受体;与正常组比较,EphA4-FC组的NG^(2+)阳性细胞数量明显增多(P<0.05)。Western blot显示,与正常组比较,EphA4-FC组细胞蛋白中PDGFαR的表达显著增高(P<0.05)。结论培养的OPC上高表达EphA4受体,通过抑制EphA4受体可以促进OPC的增殖。

Objective To investigate the effect of inhibit EphA4 receptor on oligodendrocyte progenitor cell （OPC）. Methods The purified OPC was randomly divided into normal group and EphA4-FC group （250 nmol/L, 2 d）. Immunofluorescence staining was used to observe the co-expression of NG2 （a marker for OPC） and EphA4 receptor. Cell counting was used to observe the change of OPC. Western blot was used to examine the expression of platelet-derived growth factor α receptor （PDGFαR, a marker for OPC） in two groups. Results Immunofluorescence staining showed that the high expression of EphA4 receptor on OPCs in vitro, and compared with normal group, more NG^2＋ cells were observed in the EphA4-FC group （P 〈 0.05）. Western blot showed that PDGFαR expression was significantly increased, compared with normal group （P 〈 0.05）. Conclusion EphA4 receptor is express highly on OPC in vitro, and inhibition of EphA4 receptor can promote OPC proliferation.