响应面法优化双孢菇干无硫护色剂工艺

Formula Optimization of Non-sulfur Color-protective Agents for Agaricus bisporus by Response Surface Methodology

以双孢菇为主要原料,对其无硫护色剂的组成进行了探讨。选取护色剂抗坏血酸、柠檬酸、L-半胱氨酸和柠檬酸亚锡二钠为影响因子,以双孢菇干色泽L*值为响应值。在单因素试验的基础上,采用Box-Behnken试验设计构建二次多项式回归方程的模型,进行响应面分析。结果表明:护色剂复合作用对双孢菇色泽的影响程度依次为:抗坏血酸>L-半胱酸>柠檬酸亚锡二钠>柠檬酸,复合护色剂的最优配方(质量分数)为:0.18%抗坏血酸、0.15%L-半胱氨酸、0.79%柠檬酸、0.03%柠檬酸亚锡二钠。加工的双孢菇干色泽L*值为79.83,护色效果好。

To study the color-protecting techniques of dried Agaricus bisporus, the influence of the type of color fixative was confirmed. Four components including vitamin C, citric acid, Disodium stannous citrate （DSC） and L-cysteine were used as influencing factors and the color L＊ value of dried Agadcns bispoms was used as response value. Based on one-factor-at-a- time experiments, Box-Behnken design （BBD） was applied to establish the corresponding mathematical model. The results showed that the protective effects of these four components on Agaricus bisporus color followed the decreasing order： vitamin C〉L-cysteine〉DSC〉citric acid. The optimal formula （by mass） was as followings： 0.18% vitamin C, 0.15% L-cysteine, 0.79% citric acid and 0.03% DSC. Under the optimal conditions, the L＊ value of dried Agaricus b/sporus was 79.83 indicating a good color-protecting effect.