GC-MS法测定罗汉果中有机氯农药残留量

Determination of Organochlorine Pesticide Residues in Siraitia grosvenorii by GC-MS

建立气质联用(GC-MS)法检测罗汉果中甲体-六六六、乙体-六六六、丙体-六六六、丁体-六六六、对对位滴滴依、对对位滴滴滴、邻对位滴滴涕和对对位滴滴涕8种有机氯农药残留量。样品经有机溶剂超声提取,硫酸净化,选用HP-5MS毛细管柱对8种有机氯农药进行分离,外标法计算含量。结果表明:8种有机氯农药的线性范围为5~500μg/L,相关系数均大于0.999 0,方法最低检出限为1.15~2.64μg/kg,在3个浓度水平进行加标(100,150和250μg/L),加标回收率为87.3%~109.8%,相对标准偏差(RSD,n=6)为0.30%~11.76%。该方法操作简单、结果准确、灵敏度高,可用于罗汉果中8种有机氯农药残留的测定。

A method for determining α-BHC, β-BHC, γ-BHC, δ-BHC, p, p＇-DDE, p, p＇-DDD, o, p＇-DDT and p, p＇-DDT residues in Siraitia grosvenorii using gas chromatography-mass spectrometry was developed. The pesticide residues were extracted using organic solvent. After purifying with sulfuric acid, the sample was analyzed by GC-MS with HP-5MS capillary column. The contents of 8 organochlorine pesticides were calculated by extemal stamdard. The result showed that the linear equations were obtained in the concentration range of 5-500 μg/L with correlation coefficients greater than 0.999 0. The limits of detection were 1.15-2.64 μg/kg. In 3 different degrees（100, 150 and 250 μg/L）, the recoveries were 87.3%-109.8%. The relative standard deviations were 0.30%-11.76%. The method was sensitive and accurate, and it could better meet the demand for analyzing organochlorine pesticides residues in Siraitiae Fructus.