摘要
采用自动在线衍生-高效液相色谱法测定化妆品中游离甲醛的含量。样品经乙腈涡旋提取,以2,4-二硝基苯肼的乙腈-乙酸溶液为衍生液,在线衍生2min后,衍生物在Agilent C_(18)色谱柱上分离,以乙腈(65+35)溶液为流动相进行洗脱,用二极管阵列检测器测定,检测波长为355nm。甲醛线性范围为1.0~20.0mg·L^(-1),测定下限(10S/N)为50mg·kg^(-1)。加标回收率在83.0%~98.4%之间,测定值的相对标准偏差(n=6)在3.4%~7.8%之间。
Free formaldehyde in cosmetic was determined by HPLC combined with automatm online derivatization. Free formaldehyde in cosmetic samples was extracted by acetonitrile and reacted efficiently with 2,4- dinitrophenylhydrazine in acetonitrile-acetic acid solution for 2 min. The formaldehyde derivative was separated on an Agilent C18 chromatographic column with acetonitrile-water （65 ＋ 35） as the mobile phase for elution, and then detected by DAD at the wavelength of 355 nm The linear range of formaldehyde was 1.0-20. 0 mg·kg^-1 , with the lower limit of determination （10S/N） of 50 mg·kg^-1. Recovery rates obtained by standard addition method were in the range of 83.0%-98. 4% and RSDs （n=6） were in the range of 3. 4%-7. 8%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2017年第3期281-283,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家质量监督检验检疫总局课题(2013Ik190)
浙江省科技厅项目(2013C33038)
