摘要
探讨秋葵果荚和果蒂在活性多糖提取及降血糖方面的应用。通过水提醇沉的方法,在料液比为1:35,p H值为4.5,提取温度45℃,水浴时间2 h的提取条件下确定纤维素酶的最佳用量;采用苯酚硫酸法、考马斯亮蓝法测定黄秋葵蒂和荚多糖提取物中多糖与蛋白质的含量;Sevage法去除残留蛋白,比较不同处理多糖样品对α-淀粉酶的抑制作用。在纤维素酶浓度为3%时,多糖得率最高。秋葵蒂粗提物中多糖含量(47.6%)高于果荚(36.6%)中,除去蛋白后蒂和荚对α-淀粉酶的抑制率均有所下降,分别由74.9%下降到66.5%和67.6%下降到59.5%。秋葵蒂在秋葵多糖降血糖活性方面的研究中不容忽视。
The extraction of polysaccharides from okra pod and stalk as well as the effects on α-amylase were studied in this test. The okra polysaccharides were extracted by water and precipitated by alcohol. The optimum cellulase dosage was determined. Phenol-sulfuric acid method and Coomassie brilliant blue method were used to measure polysaccharides and protein contents of okra pod and okra stalk. After using Sevage method, α-amylase inhibitory effects of four different treatments of okra polysaccharides extraction were compared. The content of polysaccharides from okra stalk were higher than that from okra pod, with 47.6% to 36.6%. Treatments of deproteinization decreased the α-amylase inhibitory ratio of okra stalk and okra pod from 74.9% to 66.5% and 67.6% to 59.5%, separately. The hypoglycemic activity of okra stalk would not be ignored in the future research.
出处
《食品科技》
CAS
北大核心
2017年第2期193-196,共4页
Food Science and Technology
基金
天津市大学生创新创业训练计划项目(201610061174)
