野生黑果枸杞花青素提取工艺优化及地区差异性

Optimization of anthocyanin extraction technology from wild Lycium Ruthenicum and differences among dfferent regions

采用响应面法优化野生黑果枸杞花青素提取工艺。以野生黑果枸杞为实验材料,在单因素实验基础上,以乙醇浓度、提取温度、提取时间和液料比为自变量,花青素提取量为响应值,通过Box-Behnken实验设计方案确定最佳提取工艺条件。结果表明,野生黑果枸杞花青素最佳提取条件为:乙醇浓度79%,提取温度37℃,提取时间68 min,料液比1:21(g/mL)。在此条件下,花青素提取量为(2.71±0.04)%。同时发现不同地区野生黑果枸杞花青素含量之间存在明显差异性,其中内蒙古额吉纳旗西部野生黑果枸杞中花青素含量最高,达2.71%,其次为内蒙古阿拉善右旗阿拉腾敖包,其花青素含量为2.57%。

The optimum extraction condition of anthocyanin from wild Lycium Ruthenicum was investigated by response surface methodology. On the basis of single factor experiments, alcohol concentration, extraction temperature, extraction time and material liquid ratio were selected for independent variable and the extraction content of anthocyanin was response value. Subsequently, the Box-Behnken experimental design was performed to optimize the extraction conditions of anthocyanin. The results indicated the optimum conditions for anthocyanin extraction were obtained as follows： alcohol concentration was 79%, extraction temperature was 37 ℃, extraction time was 68 min and material liquid ratio was 1：21（g/mL）. Under these conditions, the best extraction content of anthocyanin was（2.71±0.04）%. Meanwhile, great variation in anthocyanin content of wild Lycium Ruthenicum was found among different region. The sample from Mesozoic-cenozoic Inner Mongolia had the highest anthocyanin contents（2.71%）. Secondly for the sample from Alxa right Banner Inner Mongolia, which anthocyanin contents were 2.57%.