摘要
以橡胶草根尖和嫩叶为材料,对其染色体制片技术的取样时间、预处理试剂、解离方法及解离时间进行比较研究。结果表明:橡胶草根尖和嫩叶取样最佳时间均为上午8:00~10:00;2 mmol/L 8-羟基喹啉溶液室温预处理4 h效果最好;根尖和嫩叶用1 mol/L盐酸于60℃下分别酸解10和12 min效果良好。
The root tips and young leaves of Taraxacum kok-saghyz Rodin were used for chromosome squashing to compare the differences in chromosome squashing through sampling time, pretreatment chemical solution, hydrolysing methods and time of chromosome squashing.The results showed that the root tips and young leaves were best sampled at about 8：00 ~10：00 am, pretreated in 2 mmol/L8-hydroxyquinoline solution at a room temperature for 4 hours and hydrolysed with 1 mol/L HCl at 60℃for 10 and 12 min respectively for chromosome squashing.
作者
杨玉双
甘霖
覃碧
刘实忠
Yang Yushuang Gan Lin Qin Bi Liu Shizhong(Rubber Research Institute, CATAS, Danzhou, Hainan 571737 China Guangzhou Experimental Station, CATAS, Guangzhou, Guangdong 510140 China)
出处
《热带农业科学》
2017年第2期31-34,共4页
Chinese Journal of Tropical Agriculture
基金
橡胶研究所省部重点实验室及科学观测实验站开放课题“新型产胶作物橡胶草优异种质筛选及配套栽培技术研究与示范”(No.RRI-KLOF201604)
橡胶研究所基本科研业务费专项“重要产胶替代植物种质资源收集与鉴定”(No.1630022015005)
关键词
橡胶草
根尖
嫩叶
染色体制片技术
Taraxacum kok-saghyz Rodin
Root tip
Young leaf
Chromosome squashing technique
