有氧运动联合抗阻力运动对糖尿病大鼠血管内皮功能的作用及其对磷脂酰肌醇-3激酶/蛋白激酶B/内皮型一氧化氮合酶通路的影响

Effect of aerobic exercise combined with resistive exercise on vascular endothelial function and its impact on phosphatidylinositol 3-kinase/protein kinase B/endothelial nitric oxide synthase pathway in diabetic rats

目的探讨有氧运动联合抗阻力运动对糖尿病大鼠血管内皮功能及对磷脂酰肌醇-3激酶/蛋白激酶B/一氧化氮合酶(PI3K/Akt/eNOS)通路的影响。方法将SD大鼠随机分为对照组(NC)、糖尿病模型组(DM)、单纯有氧运动训练组(AE)、单纯抗阻力运动训练组(RE)及有氧运动+抗阻力运动训练组(ARE),检测各组内皮功能指标、PI3K/Akt/eNOS通路基因及蛋白表达情况。结果乙酰胆碱(Ach)浓度在3×10^(-5) mol/L时,ARE组舒张率高于AE组和RE组(P<0.05)。ARE组VEGF、一氧化氮(NO)表达量高于DM组[(21.45±3.48)vs(8.23±1.14)pg/ml,(25.67±3.19)vs(8.89±1.56)μmol/L,P<0.05]。ARE组PI3K、Akt及eNOS基因相对表达量高于DM组[(4.08±0.83)vs(0.27±0.11),(2.75±0.69)vs(0.26±0.09),(4.25±0.74)vs(0.59±0.19),P<0.05]。ARE组PI3K、磷酸化Akt(p-Akt)及磷酸化eNOS(p-eNOS)蛋白相对表达量高于DM组[(0.78±0.21)vs(0.20±0.09),(1.21±0.38)vs(0.54±0.19),(0.59±0.23)vs(0.16±0.08),P<0.05]。结论有氧运动联合抗阻力运动能改善糖尿病大鼠血管舒张功能,促进VEGF、NO的释放,作用与激活PI3K/Akt/eNOS信号通路有关。

Objective To investigate the effects of aerobic exercise combined with resistive exercise on vascular endothelial function and its impact on phosphatidylinositol3-kinase/protein kinase B/endothelial nitric oxide synthase（PI3K/Akt/eNOS）pathway in diabetic rats. Methods SD rats were randomly divided into control group（NC）,diabetic group（DM）,aerobic exercise training group（AE）,antiresistance exercise training group（RE）,and aerobic exercise ＋ against resistance exercise training group（ARE）.The endothelial function indicators were tested.Genes,protein expression of PI3K/Akt/eNOS pathway were investigated. Results When the concentration of Ach was 3×10-5 mol/L,the diastolic rate was significantly higher in ARE group than in AE and RE group（P 〈0.05）.VEGF and NO expression were significantly higher in ARE group than in DM group[（21.45±3.48）vs（8.23±1.14）pg/ml,（25.67±3.19）vs（8.89±1.56）μmol/L,P〈0.05].PI3 K,Akt and eNOS gene expression were relatively higher in ARE group than in DM group[（4.08±0.83）vs（0.27±0.11）,（2.75±0.69）vs（0.26±0.09）,（4.25±0.74）vs（0.59±0.19）,P〈0.05].PI3 K,p-Akt and p-eNOS protein expression were relatively higher in ARE group than in DM group[（0.78±0.21）vs（0.20±0.09）,（1.21±0.38）vs（0.54±0.19）,（0.59±0.23）vs（0.16±0.08）,P〈0.05]. Conclusion Aerobic exercise combined with resistive exercise can improve the diastolic function and promote VEGF,NO release in diabetic rats,which may be related to activation of PI3K/Akt/eNOS signaling pathway.