镇肝熄风汤对自发性高血压大鼠酪酪肽表达的影响

Influence of Zhengan Xifeng Decoction on the Expression of Peptide Tyrosine Tyrosine in Spontaneously Hypertensive Rats

目的探讨镇肝熄风汤对自发性高血压大鼠(SHR)酪酪肽(PYY)表达的影响。方法 2012年6月—2015年4月选取14周龄雄性SHR 75只,随机分为模型组(n=15)、贝那普利组(盐酸贝那普利灌服0.90 mg·kg^(-1)·d^(-1),n=15)、镇肝熄风汤低、中、高剂量组(镇肝熄风汤分别灌服15、30、60 g·kg^(-1)·d^(-1),n=15),以WKY大鼠15只作为WKY组,模型组及WKY组每日灌服同体积的蒸馏水。干预8周后,实验过程中WKY组大鼠死亡6只,模型组大鼠死亡6只,贝那普利组大鼠死亡7只,镇肝熄风汤高剂量组大鼠死亡6只,镇肝熄风汤中剂量组大鼠死亡6只,镇肝熄风汤低剂量组大鼠死亡6只应用酶联免疫实验法检测各组大鼠血清PYY水平,采用免疫组化法检测结肠组织PYY水平,RT-PCR法检测结肠组织PYY mRNA水平。结果模型组血清PYY水平高于WKY组(P<0.05);镇肝熄风汤高剂量组血清PYY水平低于模型组(P<0.05)。模型组结肠组织PYY水平高于WKY组(P<0.05);贝那普利组和镇肝熄风汤高、中、低剂量组结肠组织PYY水平低于模型组(P<0.05);镇肝熄风汤高剂量组结肠组织PYY水平低于贝那普利组(P<0.05)。模型组结肠组织PYY mRNA水平高于WKY组(P<0.05);贝那普利组和镇肝熄风汤高、中剂量组结肠组织PYY mRNA水平低于模型组(P<0.05);镇肝熄风汤高剂量组结肠组织PYY mRNA水平低于贝那普利组(P<0.05)。结论 SHR结肠组织PYY调节失衡,镇肝熄风汤可能通过调节PYY的表达而达到降压目的。

Objective To investigate the influence of Zhengan Xifeng decoction on the expression of peptide tyrosine tyrosine（ PYY） in spontaneously hypertensive rats（ SHRs）. Methods We randomly assigned 75 SHRs（ 14 weeks old,male） into model group（ n = 15）,Benazepril group（ n = 15）,Zhengan Xifeng decoction high,medium,and low dose groups（ 15 SHRs in each group）,and set 15 WKY rats as the WKY group between June 2012 and April 2015. During the intervention period of 8 weeks,six rats died in model group,WKY group,Zhengan Xifeng decoction high,medium,and low dose group,seven rats died in Benazepril group. The rats in model group and WKY group were given the same volume of distilled water every day,Benazepril group were fed with Benazepril 0. 90 mg·kg^-1·d^-1Zhengan Xifeng decoction high,medium,and low dose groups were fed with Zhengan Xifeng decoction 15,30,60 g·kg^-1·d^-1respectively. At the end of intervention,the level of PYY in serum was detected by ELISA,the expression of PYY and its mRNA in colon was detected by immunohistochemistry and RT- PCR. Results The serum PYY levels in model group were significant higher than those in WKY group（ P〈0.05）.Zhengan Xifeng decoction high dose group had lower serum PYY levels than model group（ P〈0.05）. Compared with the model group,the expression of PYY in colon was lower in the WKY group（ P〈0.05） as well as in Benazepril group,and Zhengan Xifeng decoction high,medium,and low dose groups（ P〈0.05）. The expression of PYY in colon in Zhengan Xifeng decoction high dose group was lower than that in Benazepril group（ P〈0.05）. WKY group had lower expression of PYY mRNA in colon than model group（ P〈0.05）, likewise, Benazepril group, and Zhengan Xifeng decoction high, medium dose groups possessed lower expression of PYY mRNA in colon than model group（ P〈0.05）. Zhengan Xifeng decoction high dose group displayed a lower expression of PYY mRNA in colon in comparison to Benazepril group（ P〈0.05）. Conclusion SHRs have regulation disorder of PYY in colon. The antihypertensive effect of Zhengan Xifeng decoction may be realized by regulating the expression of PYY.