基质金属蛋白酶-2与丙型肝炎及丙型肝炎肝硬化的相关性

Matrix Metalloproteinase-2 correlates with Hepatitis C virus infection and HCV induced liver cirrhosis

目的探讨血清基质金属蛋白酶-2（Matrix Metaltoproteinase-2，MMP-2）与丙型肝炎及丙型肝炎肝硬化的相关性。方法选择42例丙型肝炎患者、96例丙型肝炎肝硬化患者和30例健康者参与本次研究。使用酶联免疫法测量血清MMP-2水平，对比分析3组间MMP-2水平差异；采用Chfld-Pugh评分系统对丙型肝炎肝硬化组患者进行分级，分析MMP-2水平同Child-Pugh评分的相关性。结果MMP-2水平丙型肝炎组（80．26±12．96ng／mL）和丙型肝炎肝硬化组（114．61±32．72ng／mL）高于健康对照组（65．32±10．24ng／mL），差异具有统计学意义（P〈0．05）；丙型肝炎肝硬化患者随着Child～Pugh分级升高，MMP-2水平呈显著上升趋势，线性分析显示，MMP-2水平与Child—Pugh评分呈正相关（r=0．66，P〈0．05）。结论MMP-2水平与丙型肝炎、丙型肝炎肝硬化病情发展密切相关，与丙型肝炎肝硬化肝脏损伤程度正相关。检测MMP-2水平可辅助评估丙型肝炎患者肝脏受损情况，预测肝纤维化程度。

Objectives This study was aimed to investigate the correlation of plasma level of matrix metalloproteinase-2 （MMP-2） in patients with hepatitis C virus （HCV） and HCV in- duced cirrhosis. Methods Participants were comprised of 42 HCV patients, 96 HCV induced liver cirrhosis patients and 30 healthy individuals. Serum levels of MMP-2 were measured with enzyme-linked immunosorbent assay. Child-Pugh scoring system was used to categorize hepatic dysfunction in HCV induced cirrhosis patients. Results Serum levels of MMP2 were significantly increased in HCV infection（80.26＋12.96 ng/mL） and liver cirrhosis （114.61±32.72ng/mL ng/ mL）（P〈0.05）, compared to control group （65.32±10.24 ng/mL）. The level of MMP-2 in patients with HCV induced cirrhosis increased significantly with the Child-Pugh classification. Linear analysis revealed that the MMP-2 level was positively correlated with the severity of liver damage according to Child Pugh results（r=0.66, P〈0.05）.Conclusions Serum MMP-2 level, correlated to the disease progression in HCV infection and HCV induced cirrhosis. Serum MMP-2 level may be as a useful non-invasive progression marker for the surveillance of disease development and predication of cirrhosis severity in patients with HCV infection.