CXCL12,CXCR4及CXCR7表达检测对乳腺癌患者疾病预后意义探讨

Prognostic value of detecting the expression of CXCL12,CXCR4,and CXCR7 in breast cancer patients

目的探讨乳腺癌患者肿瘤组织中CXCL12,CXCR4和CXCR7 m RNA表达情况在肿瘤转移和疾病预后中的价值。方法采用定量PCR方法检测115例乳腺癌,临近正常组织及乳腺癌肿瘤转移患者颈部淋巴结样本中CXCL12,CXCR4和CXCR7 m RNA表达情况。随访资料采用Kaplan-Meier生存分析,对影响生存质量的因素进行多重变量Cox回归分析。结果与正常组织相比,乳腺癌组织中CXCR4和CXCR7表达明显增加,差异均有统计学意义(P﹤0.001),两种组织中CXCL12的表达差异无统计学意义(P﹥0.05);CXCL12在肿瘤原发部位和淋巴结转移部位的表达差异有统计学意义(P﹤0.05),转移瘤的CXCR4和CXCR7表达均增加(P﹤0.05)。KaplanMeier生存分析结果表明,与CXCR4和CXCR7低表达患者相比,高表达患者的总生存率较低(P﹤0.05)。Cox回归模型显示,CXCL12、CXCR4和CXCR7表达均为影响乳腺癌患者生存情况的独立因素。结论本研究结果表明CXCL12、CXCR4和CXCR7 m RNA表达在乳腺癌患者肿瘤发展和转移中发挥重要作用,可以作为乳腺癌患者疾病预后的生物标志物。

Objective To explore the predictive value of detecting the expression of CXCL12, CXCR4 and CXCR7 m RNA in the metastasis and prognosis of breast cancer patients. Method Quantitative PCR was used to detect the CXCL12, CXCR4 and CXCR7 m RNA expression in specimens of cervical lymph node metastasis of 115 cases of primary breast cancer, and in normal tissues and breast cancer metastasis. Survival time of 115 patients was estimated by KaplanMeier survival curves using log-rank test. Independent prognostic factors for survival were accessed by using multivariable Cox regression analysis. Result Compared with adjacent normal tissues, the expression levels of CXCR4 and CXCR7 in breast cancer tissues were significantly higher（P〈0.001）, but CXCL12 expression were similar（P〈0.05）; Additionally, CXCL12 expression was significantly different in lymph node metastasis and primary tumor tissues（P〈0.05）,and the expression of CXCR4 and CXCR7 in metastasis were significantly increased（P〈0.05）. Kaplan- Meier survival analysis indicated that, patients with higher CXCR4 and CXCR7 expression showed a shorter survival time compared with those with lower CXCR4 and CXCR7 expression（P〈0.05）. Cox regression model analysis demonstrated that expression of CXCL12, CXCR4 and CXCR7 were independent prognostic factors for overall survival in patients with breast cancer. Conclusion The CXCL12, CXCR4 and CXCR7 m RNA expressions are essential in the metastasis and progression of breast cancer, which may serve as predictive biomarkers in evaluating patients＇ prognosis.