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基于适配体技术的雌性激素检测方法研究进展 被引量:7

Research on Aptasensor Development for Estrogen Detection
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摘要 雌激素是一种类固醇激素,属于环境内分泌干扰物,可在人体和动物体内积累并起到类似雌激素的作用,使生物体的内分泌失衡,因此开发简单快速灵敏的雌激素检测方法具有重要意义。核酸适配体是通过指数富集配体系统进化技术(SELEX)筛选得到的一条寡核苷酸序列,能够与靶标高亲和力特异性结合,与传统抗体相比,具有靶标范围广、易合成修饰、稳定性高等特点,因而被广泛用于分析检测领域。该文主要综述了雌激素适配体的筛选进展以及适配体技术在雌激素检测中的应用,并对核酸适配体技术在雌激素检测领域的应用前景进行了展望。 Estrogen is a kind of steroid hormone, belonging to endocrine disruptors, which seriously interfere with the normal endocrine function of human and wildlife. Therefore, it is quite necessary to develop a simple and rapid method for the sensitive determination of estrogen present in the environment to protect public and environment health. Aptamers are selected in vitro by systematic evolution of ligands by exponential enrichment(SELEX) , and are single-stranded DNA or RNA molecules that can bind to various targets with high sensitivity and specificity. Aptamer has been widely used in a- nalysis field because of its several overwhelming advantages over antibodies, such as target diversity, high stability, easy of synthesis and modification for applications. In this review, the researches on estrogen aptamer selection and aptamer based detection methods were summaried, and the development trend of aptasensor application in estrogen detection was also discussed.
作者 张桂兰 朱超 黄亚飞 闫娇 陈爱亮 ZHANG Gui-lan ZHU Chao HUANG Ya-fei YAN Jiao CHEN Ai-liang(Institute of Quality Standards and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
出处 《分析测试学报》 CAS CSCD 北大核心 2017年第3期422-430,共9页 Journal of Instrumental Analysis
基金 公益性行业(农业)科研专项资助(201203046)
关键词 雌激素 适配体 比色法 荧光法 电化学 综述 estrogen aptamer colorimetry fluorescence electrochemistry review
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