脾多肽对急性肺损伤大鼠TLR9信号相关因子的影响

Influenc of Spleen Polypeptide on TLR9 Signal Related Factors in Rats with Acute Lung Injury

目的:分析脾多肽对急性肺损伤(ALI)大鼠Toll样受体9(TLR9)信号相关因子的影响。方法:60只无特定病原体级(SPF)Wistar大鼠,40只采用BIM-Ⅱ型水平式生物撞击机持续撞击右侧胸部复制ALI大鼠模型,造模成功后的大鼠均分为脾多肽组(n=20,经耳静脉注射5mg/Kg脾多肽)及ALI组(n=20,经耳静脉注射等量生理盐水);20只作为对照组(经耳静脉注射等量生理盐水),注射6 h后处死大鼠,HE染色观察3组大鼠肺组织病理学改变,ELISA法检测大鼠灌洗液中肿瘤坏死因子(TNF-α)、白细胞介素-8(IL-8)和白细胞介素-6(IL-6)的水平,RT-PCR法检测肺组织中NF-κB、髓样分化因子(Myd88)、Toll样受体(TLR9)mRNA的水平。结果:HE染色显示,ALI与脾多肽组均出现了ALI表现;与对照组比较,ALI组和脾多肽组大鼠TNF-α、IL-8及IL-6蛋白表达水平,NF-κB、Myd88及TLR9 mRNA的表达均增加,脾多肽组低于ALI组,差异有统计学意义(P<0.05)。结论:脾多肽可显著下调ALI大鼠TLR9信号相关因子表达水平,降低ALI程度。

Objective：To investigate the effect of spleen polypeptide on Toll-like receptor 9（ TLR9）signal-related factors in rats with acute lung injury（ ALI）. Methods：Sixty specific pathogen free Wistar rats were used as the research object,of which 20 were as the control group（ by ear intravenous injection of normal saline at 5 mg/Kg,group CON）,and the other 40 rats were prepared as ALI model by continuously striking with a BIM-Ⅱ type biological impact machine on the right chest. Then,the model rats were divided into ALI group（ by ear intravenous injection normal saline at 5 mg/Kg）（ group ALI） and spleen polypeptide group（ by ear intravenous injection of spleen polypeptides at 5 mg/Kg）（ group SPP）. In 6 h after injection the rats were sacrificed,and lung histopathological changes were observed with HE staining technique,tumor necrosis factor（ TNF-α）,interleukin-8（ IL-8） and interleukin-6（ IL-6） in rat lavage fluid were assayed by ELISA,and the changes of NF-κB,Myd88 and Toll-like receptors（ TLR9） mRNA in lung tissue were detected by RT-PCR. Results：HE staining observation showed ALI characteristics in groups ALI and SPP. Levels of TNF-α,IL-8,IL-6,and expression of NF-κB,Myd88,and TLR9 mRNA were higher in groups ALI and SPP than in group CON.And all of those indexes were higher in group ALI than in group SPP. The differences were statistically significant（ P〈0. 05）. Conclusion：Spleen polypeptide can significantly down-regulate the TLR9 signal-related factors in ALI rats,and reduce the degree of lung injury.