基因多态性与超声波活体测定相结合对肉牛主要肉质性状的研究

Study on Genetic Polymorphism and Ultrasound Measurements for Major Meat Quality Traits in Beef Cattle

本试验采用PCR-SSCP与超声波活体测定相结合的方法,对Leptin基因外显子ⅡE2JW的SNP位点与宰前/宰后新疆褐牛背膘厚度、眼肌面积、脂肪含量、眼肌高度的部分肉质性状进行关联性分析。结果表明,外显子ⅡE2JW位点的PIC处于中度多态(0.25<PIC<0.5),E2JW多态位点存在3个基因型:AA、AB和BB,其中AB为优势基因型,B为优势等位基因。相关性结果表明,除预测值中AB、AA基因型个体的肌肉脂肪含量与实测值的分析结果不一致外,其他均表现出新疆褐牛Leptin基因外显子ⅡE2JW多态性与预测肉质性状和实测肉质性状关系的一致性。因此,Leptin基因外显子ⅡE2JW是影响新疆褐牛肉质性状的候选基因位点,基因多态性结合超声波活体测定技术不仅可以快速、准确地测量部分肉质性状,而且可作为分子标记辅助选择的一种手段。

In this study,we used PCR-SCCP connect with ultrasonic vivo assay techniques,analysis of correlation between Leptin gene exonⅡ E2 JW polymorphism and part meat quality traits of backfat thickness,eye muscle area,fat content,plot eye height in Xinjiang Brown cattle at ante-mortem,postmortem.The results showed that Leptingene exonⅡE2JW site PIC was moderate polymorphisms（0.25〈PIC〈0.5）,there were three genotypes of AA,AB and BB,AB genotype were the predominant genotype,B was the predominant allele.In addition,the forecast value of AB and AA gennotypes individual muscle fat content were inconsistent with actual value.Leptingene exonⅡE2JW was identified the theroy of a candidate gene loci of beef quality traits in Xinjiang Brown cattle.Genetic polymorphism and ultrasound measurements could not only fastly and accurately measure portion meat traits,but also as a means of marker-assisted selection.