新疆猪源沙门氏菌耐药性及耐药基因检测

Detection of Resistance and Resistance Genes of Salmonellafrom Swine in Xinjiang

为了解新疆某规模化养殖场猪源沙门氏菌对临床上常用抗菌药物的耐药情况,以及β-内酰胺酶、16S rRNA甲基化酶和质粒介导的喹诺酮耐药(plasmid mediated quinolone resistance,PMQR)基因的流行情况,本试验通过琼脂稀释法对分离的菌株进行最小抑菌浓度测定,PCR方法进行β-内酰胺酶blaTEM、blaCMY-2、blaCTX-M、blaLAP-1、blaKPC、blaOXA和blaSHV基因,16SrRNA甲基化酶基因armA和rmtB及PMQR类基因qnrA、qnrB、qnrC、qnrD、qnrS、qepA、oqxA、oqxB和aac(6′)-Ib-cr的检测,确定阳性菌株,分析其携带的基因型与耐药表型之间的关系。分离的猪源沙门氏菌对环丙沙星和安普霉素耐药率最高,均为77.9%(102/131),耐药菌主要以8耐为主(29.0%,38/131)。从被检基因中检测出11种耐药基因,不同基因共存有24种类型,主要以blaTEM+blaOXA+qnrS+aac(6′)-Ib-cr+oqxA+oqxB(27.6%,32/116);blaTEM+blaOXA+qnrS+oqxA+oqxB(24.1%,28/116);blaTEM+qnrS(18.0%,21/116)形式共存。该养殖场分离的沙门氏菌耐药现象严重,分离耐药菌株存在β-内酰胺酶、16SrRNA甲基化酶和PMQR类基因共存现象,提示应加强对β-内酰胺酶、16SrRNA甲基化酶和PMQR类因子的监控。

The objective of this study was to investigate the resistance of Salmonellaand prevalence of resistance genes isolated from a pig farm in Xinjiang,and their coexistence with the majorβ-lactamases,16 SrRNA methylation enzyme genes and PMQR.The minimum inhibitory concentrations of Salmonellaisolated from the pig farms were determined by agar dilution method,PCR was used to detect blaTEM,blaCMY-2,blaCTX-M,blaLAP-1,blaKPC,blaOXAand blaSHVgenes,16 SrRNA methylation enzyme genes armA and rmtB,and PMQR including qnrA,qnrB,qnrC,qnrD,qnrS,qepA,oqxA,oqxBand aac（6′）-Ib genes.The positive strains were performed by using DNA sequencing to determine the purpose of the belt.The result showed that resistant rate of Salmonellaisolates from swine were highest to ciprofloxacin and apramycin sulfate（77.9%,102/131）.Resistant mainly based on 8kinds of drug resistance（29.0%,38/131）,11 kinds of resistance genes were detection,the coexistence of different genotypes had 24 types.The main types were blaTEM ＋blaOXA ＋qnrS＋aac（6′）-Ib-cr＋oqxA＋oqxB（27.6%,32/116）,blaTEM＋blaOXA＋qnrS＋oqxA＋oqxB（24.1%,28/116）and blaTEM＋qnrS（18.0%,21/116）.The Salmonellaisolated from the farm had phenomenon seriously resistance,it coexisted with the mainβ-lactamase,16 SrRNA methylation enzyme genes and PMQR factors.The result suggested that it should strengthen monitoring to theβ-lactamase enzymes,16 SrRNA methylation enzyme genes and PMQR factors.