解淀粉芽孢杆菌SSY1株的安全性试验

Evaluation of Safety of Bacillus amyloliquefaciens SSY1

为评估1株产纤维素酶的解淀粉芽孢杆菌SSY1株的安全性,本试验对其进行了急性毒性、慢性毒性、细菌易位、代谢产物及药敏等安全性试验。急性毒性试验分为高剂量组(1.30×1010 CFU/mL)、中剂量组(0.90×1010 CFU/mL)、低剂量组(0.65×1010 CFU/mL)和生理盐水对照组,一次性灌服后观察14d,处死存活小鼠,剖检。慢性毒性试验分为高剂量组(0.65×1010 CFU/mL)、中剂量组(0.65×109 CFU/mL)、低剂量组(0.65×108 CFU/mL)和生理盐水对照组,各组灌服1次/d,连续灌服30d。结果表明,急性毒性试验小鼠的精神、食欲、行为、粪便等均未见异常,试验鼠全部存活,剖检未见病理变化;慢性毒性试验小鼠均无异常临床变化,剖检小鼠也未见病变,高、中、低剂量组及对照组间小鼠增重、饲料利用率、血液学指标、血液生化指标及脏器系数均差异不显著(P>0.05);解淀粉芽孢杆菌SSY1菌株在小鼠体内未发生易位,氨基脱羧酶、吲哚试验均为阴性,在测定的24种常用药物中,除林可霉素耐药外,菌株对其余23种药物均敏感。试验证实,解淀粉芽孢杆菌SSY1株的安全性良好。

In order to determine the safety of Bacillus amyloliquefaciens SSY1,we carried out the acute toxicity,chronic toxicity,bacterial translocation,product of metabolism and drug susceptibility test.Acute toxicity test in high dose groups（1.30×10^10 CFU/mL）,middle dose group（0.90×10^10 CFU/mL）,low dose group（0.65×10^10 CFU/mL）and normal saline control group.After the acute toxicity test,the mice were sacrificed 14 days after the observation.Chronic toxicity test of each group was administered once a day for 30 d,chronic toxicity test in high dose groups（0.65×10^10 CFU/mL）,middle dose group（0.65×10^9 CFU/mL）,low dose group（0.65×10^8 CFU/mL）and normal saline control group.The results showed that all mice were without exception phenomenon,the spirit,appetite,behavior and feces and so on,no pathological changes were found on the acute toxicity test.Chronic toxicity test of the mice were no abnormal clinical changes,and there were no pathological changes.Body weight and feed utilization rate of mice,hematology,blood biochemistry indexes and viscera coefficient difference were not significant（P〉0.05）;Bacillus amyloliquefaciens SSY1 strain did not occur translocation in mice,amino acid decarboxylase and indole test were negative,among the 24 kinds of commonly used drugs,the strains were sensitive to 23 kinds of drugs except the cillimycin.This experiment proved that Bacillus amyloliquefaciens SSY1had good security.