复方地黄合剂及复方逍遥合剂中黄芩苷含量测定

Determination of baicalin content in both compound Dihuang and compound Xiaoyao oral solutions

目的 :建立复方地黄合剂及复方逍遥合剂中黄芩苷含量的测定方法。方法 :采用高效液相色谱(HPLC)法、C_(18)色谱柱(4.6 mm×250 mm,5μm)、流速1.2 ml/min,柱温25℃,波长280 nm测定,流动相为乙腈-0.2%磷酸溶液(27∶73)。结果 :在不同中药制剂中,黄芩苷峰形良好,方法专属性强。在浓度为16~320μg/ml范围内均呈良好的线性关系(r=0.999 3);在50%、100%、150%的浓度下,二制剂中黄芩苷的回收率分别为99%及97%,RSD分别为1.7%和1.6%。此外,黄芩苷在8 h内含量变化的RSD分别为0.3%和0.2%,稳定性良好。结论:该方法简便、准确、灵敏度高、重复性好,可用于复方地黄合剂及复方逍遥合剂中黄芩苷的含量的测定。

Objective： To establish a method for the determination of baicalin content in both compound Dihuang and compound Xiaoyao oral solutions. Methods： The established HPLC method was carded out on a C18 column at detection wavelength of 280 nm with a mobile phase consisted of acetonitrile-0.2% phosphoric acid solution （27 ： 73）. Results： The peak shape of baicalin in different preparations of traditional Chinese medicine and the specificity of the method were good. The standard curve of baicalin showed a good linear relationship over the range of 16- 320 μg/ml （r=0.999 3）. The average recoveries were 99% with RSD 1.7% for compound Dihuang oral solution and 97% with RSD 1.6% for compound Xiaoyao oral solution under the concentrations of 50%, 100%, 150% of original ones. Besides, the RSD of baicalin content change was 0.3% and 0.2% in 8 hours of storage, indicating its good stability. Conclusion： This method is convenient and accurate with high sensitivity and good reproducibility, can be used to determine the content of baicalin in both compound Dihuang and compound Xiaoyao oral solutions.