白芷种子蛋白质提取方法的比较

Comparison of Protein Extraction Methods for Angelica dahurica Seeds

[目的]建立适用于白芷种子的SDS-PAGE蛋白质样品提取方法。[方法]比较5种提取方法所获得白芷种子总蛋白质产量以及SDS-PAGE电泳的谱带数目、强度和电泳分辨率等方面的差异。[结果]方法 a所提蛋白质含量高,杂质少,电泳谱带清晰;方法 b所提蛋白质溶液电泳谱带清晰,分辨率较高,但提取含量较低;方法 c和d所提蛋白质杂质较多,纯度不高,分辨率较低;方法 e所提蛋白质纯度较高,但蛋白条带缺失较多。[结论]方法 a提取白芷种子蛋白质的效果最好,所提蛋白效率较高,杂质少,电泳谱带清晰,分辨率高,谱带数多,优于其他提取法。

[ Objective ] To establish a suitable protein extraction protocol for total proteins extraction from Angelica dahurica seeds. [ Method ] We compared the total protein yield and SDS-PAGE eleetrophoretic band number and strength, leetrophoresis resolution by five different methods. [ Result] Method a had higher protein content, less impurity and clearer electrophoresis band. Method b had clearer eleetrophoresis band, higher resolution, but lower protein content. Methods c and d had more impurity, lower purity and resolution. Method e had higher protein purity, but more lack of protein bands. [ Conclusion] Method a has the best extraction effects of protein from A. dahurica seeds, which has higher protein extraction efficiency, less impurity, clearer band, higher resolution, more band number. Thus, method a is superior to other four methods.