摘要
[目的]测定核桃青皮80%乙醇提取物及其经大孔树脂HP-20分段后的各纯化部分的总酚含量,评价其各部分的抗氧化能力,并分析总酚含量与抗氧化能力的关系。[方法]采用福林-酚比色法,测定总酚含量,通过DPPH·和ABTS·自由基清除法评价其抗氧化能力。[结果]核桃青皮提取物及其各纯化部分均表现出一定的抗氧化性,其中Fr.2(20%甲醇洗脱部分)、Fr.3(40%甲醇洗脱部分)部分的IC50(半数抑制浓度)均高于VC(抗坏血酸),Fr.4(60%甲醇洗脱部分)和Ext.(80%乙醇提物部分)与VC相当,并且总酚含量和抗氧化能力呈正相关。[结论]经过大孔树脂HP-20处理后的核桃青皮乙醇提取物,其总酚的含量得到了富集,为进一步研究核桃青皮各部分的物质成分提供了依据。同时表明核桃青皮的提取物可以作为天然的抗氧化剂应用于食品、医疗、化妆品、保健品等行业。
[ Objective ] To determine the content of total phenols in the 80% ethanol extracts of walnut green husk and its several purification sections isolated and purified by HP-20, evaluate the antioxidant capacity of the extracts, and analyze the relationship between the content of polyphenols and antioxidant capacity. [ Method] The content of total phenolswere determined by the method of Folin-phenol, and its extract antioxidant capacities were compared by DPPH · and ABTS ·radical scavenging. [ Result ] All the extracts showed antioxidant activity,in which the IC50 (half maximal inhibitory concentration) value of Fr. 2( the elution fraction of 20% methanol ) and Fr. 3 ( the elution fraction of 40% methanol ) was better than that of VC (ascorbic acid), Fr. 4 (the elution fraction of 60% methanol ) and Ext. (the extract portion of 80% ethanol) was similar to VC,and polyphenol content and antioxidant capacity was positive correlation. [ Conclusion ] The content of polyphenols in the ethanol extract of walnut green husk can be enriched by HP-20 ,which will provide a basis for the further study of the material composition of the walnut green husk. The extract of walnut green husk can be used as a natural antioxidant used in food,health care, cosmetics,health products and other industries.
作者
魏欢
杨建文
颜小捷
卢凤来
WEI Huan YANG Jian-wen YAN Xiao-jie et al(Guilin University of Technology, Guihn, Guangxi 541006 Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin, Guangxi 541006)
出处
《安徽农业科学》
CAS
2017年第6期121-123,共3页
Journal of Anhui Agricultural Sciences
基金
广西壮族自治区八桂学者专项经费
广西植物研究所基本业务费(桂置业15011)
广西植物功能物质研究与利用重点实验室主任基金项目(ZRJJ2015-14)
关键词
核桃青皮
总酚
含量测定
抗氧化
Walnut green husk
Total phenols. Content detemination
Antioxidant