口腔癌患者外周血细胞免疫水平与临床结局的相关性分析

Correlation Analysis of Immune Cell Levels of Peripheral Blood and Clinical Outcomes in Patients with Mouth Cancer

目的探讨口腔癌患者外周血细胞免疫水平与临床结局的相关性。方法选取2014年1月—2015年1月我院收治的口腔癌50例(口腔癌组),选择同期体检健康的50例(对照组)。比较两组外周血淋巴细胞(CD3^+、CD4^+、CD8^+、CD19^+)和自然杀伤(natural killer,NK)细胞的水平,分析口腔癌患者外周血淋巴细胞(CD3^+、CD4^+、CD8^+、CD19^+)、NK细胞与其临床特征的相关性。结果与对照组比较,口腔癌组CD3^+及CD4^+T淋巴细胞、CD4^+/CD8^+和NK细胞显著降低(P<0.05),CD19^+T淋巴细胞显著升高(P=0.000),CD8^+T淋巴细胞降低不明显(P>0.05)。与Ⅲ~Ⅳ期口腔癌患者比较,Ⅰ~Ⅱ期患者的CD3^+、CD4^+及CD8^+T淋巴细胞、CD4^+/CD8^+和NK细胞显著升高(P<0.05),CD19^+T淋巴细胞显著降低(P=0.000)。与无淋巴结转移的患者比较,出现淋巴结转移的患者CD3^+、CD4^+及CD8^+T淋巴细胞和NK细胞显著降低(P<0.05),CD19^+T淋巴细胞显著升高(P=0.000),CD4^+/CD8^+降低不明显(P>0.05)。与无复发患者比较,术后复发者CD3^+、CD4^+及CD8^+T淋巴细胞、CD4^+/CD8^+和NK细胞显著降低(P<0.05),CD19^+T淋巴细胞显著升高(P=0.000)。结论口腔癌患者存在一定程度的免疫抑制,且外周血细胞免疫水平与其预后密切相关。

Objective To investigate correlation between immune cell levels of peripheral blood and clinical outcomes in patients with month cancer. Methods A total of 50 patients with month cancer admitted during January 2014 and January 2015 were selected as month cancer group, and 50 healthy people taking medical examination were chosen as control group. Levels of peripheral blood lymphocytes （CD3 ＋ , CD4＋ , CD8＋ and CD19 ＋） and natural killer （NK） cells were compared, and correlations between levels of peripheral blood lymphocytes （ CD3＋, CD4＋ , CD8＋ and CD19 ＋ ） and NK cell and clinical characteristics were analyzed in two groups. Results Compared with those in control group, levels of CD3 ＋ and CD4 ＋ T lymphocyte, CD4 ＋/CD8＋ and NK lymphocyte were significantly decreased （P 〈 0.05 ） ; CD19 ＋ T lymphocyte level was sig- nificantly increased （P = 0. 000） ; while CD8 ＋T lymphocyte level was not significantly decreased in month cancer group （P 〉0. 05）. Compared with those in patients with stage Ⅲ-Ⅳ, levels of CD3 ＋ , CD4＋ and CD8＋ T lymphocyte, CD4＋/CD8 ＋ and NK lymphocyte were significantly increased （P 〈 0. 05） ; CD19＋T cell level was significantly decreased in patients with stage Ⅰ-Ⅱ （P = 0. 000）. Compared with those in patients without lymph node metastasis, levels of CD3＋ , CD4＋ and CD8 ＋ T lymphocyte and NK cell level were significantly decreased （ P 〈 0. 05 ） , while CD19＋ T cell level was significantly increased （P =0. 000）, but CD4＋/CD8＋ level was not significantly decreased in patients with lymph node metastasis （P 〉 0.05）. Compared with those in patients without recurrence, levels of CD3＋ CD4 ＋ and CD8 ＋ T lymphocyte, CD4＋/CD8＋ and NK lymphocyte were significantly decreased （P 〈 0.05 ） and CD19＋T lymphocyte level was significantly increased in patients with postoperative recurrence （P = 0. 000）. Conclusion Patients with month cancer have a certain degree of immune suppression, and immunity cell levels of peripheral blood is closely associated with clinical prognosis.