肾透明细胞癌患者血清miR-214的表达及临床意义

Expression and Clinical Significance of Serum miR-214 in Patients with Clear Cell Carcinoma of Kidney

目的探讨血清miR-214在肾透明细胞癌患者中的表达及在诊断、治疗及预后判断方面的临床意义。方法选择我院确诊的肾透明细胞癌30例(肾癌组),选取同期在我院体检健康的30例(对照组)。对照组随机检测血清miR-214水平,肾癌组于术前、术后1周及术后1个月检测血清miR-214变化,比较组间的表达差异及其与临床参数的关系。结果肾癌组术前、术后1周与对照组血清miRNA-214相对表达量比较差异有统计学意义(P<0.05);肾癌组术前与术后1个月、术后1周与术后1个月之间比较差异均有统计学意义(P<0.05);肾癌组术前与术后1周、术后1个月与对照组之间比较差异无统计学意义(P=0.318,P=0.383)。利用受试者工作特征曲线做鉴别检验,获得曲线下面积(AUC)=0.858[95%CI(0.756,0.960),P<0.05],根据Youden指数找出最佳诊断点为2.91,敏感度为80.0%,特异度为90.0%。结论血清miR-214与肾透明细胞癌有明显相关性,可作为该病的潜在生物标记物。

Objective To investigate expression of serum miR-214 in patients with clear cell carcinoma of kidney and its clinical significance in diagnosis, therapy and prognosis judgement. Methods A total of 30 patients with clear cell carci- noma of kidney were selected as renal cancer group, and 30 healthy people taking medical examination at the same period were chosen as control group, and miR-214 expressions were detected by randomized method in control group, and the expressions were detected before surgery, 1 week and l month after surgery in renal cancer group, and then expression differences and cor- relations with clinical indexes were compared between two groups. Results The differences of relative miR-214 expressions before and 1 week after surgery in renal cancer group and in control group were statistically significant （ P 〈 0. 05 ）. The differ- ences of miR-214 expressions were statistically significant between before surgery and l month after surgery, and between 1 week after surgery and 1 month after surgery in renal cancer group （P 〈 0.05）. The differences of miR-214 expressions were not statistically significant between before and 1 week after surgery in renal cancer group, and between 1 month after surgery in renal cancer group with in control group （P = 0. 318 ,P = 0. 383 ）. Diagnostic test was performed by using receiver operating curve （ROC）, and area under curve （AUC） was0.858 [95%CI （0.756, 0.960）, P〈0.051. The best diagnostic point, sensitivity and specificity were 2.91, 80.0% and 90.0% respectively according to Youden index. Conclusion Expressions of serum miR-214 in patients with clear cell carcinoma of kidney have correlation with clear cell carcinoma of kidney, and therefore it can be used as a potential molecular marker.