西黄丸挥发油联合人绒毛膜促性腺激素对乳腺癌大鼠免疫功能的影响

Effects of volatile oil of Xihuang pill combined with HCG on immune function in rats with breast cancer

目的研究西黄挥发油联合人绒毛膜促性腺激素(HCG)对乳腺癌大鼠免疫功能的影响。方法将84只成功造模的乳腺癌大鼠随机分为3组,每组28只。A组给予0.9%氯化钠注射液灌胃+0.9%氯化钠注射液腹腔注射,B组给予西黄丸挥发油灌胃+0.9%氯化钠注射液腹腔注射,C组给予西黄丸挥发油灌胃+HCG腹腔注射,3组大鼠均连续干预21 d。分别收取干预前后各组大鼠静脉血测定免疫学相关指标,并于干预结束后杀死大鼠称取瘤质量并计算抑瘤率。结果 3组大鼠干预后血清CD3^+、CD4^+、CD4^+/CD8^+、自然杀伤细胞(NK)、IgG、IgM、IgA、IL-2、IL-4均较干预前明显降低(P均<0.05),CD8^+、IL-6、IL-12均较干预前明显升高(P均<0.05);B、C组干预后血清CD3^+、CD4^+、CD4^+/CD8^+、NK、IgG、IgM、IgA、IL-2、IL-4均明显高于A组(P均<0.05),血清CD8+、IL-6、IL-12均明显低于A组(P均<0.05),且C组各指标改善情况均明显优于B组(P均<0.05)。干预后B、C组瘤质量明显低于A组(P均<0.05),B组瘤质量明显低于C组(P<0.05),C组抑瘤率明显高于B组(P<0.05)。结论西黄丸挥发油能明显改善乳腺癌大鼠免疫功能,有明显抗肿瘤活力,但与HCG联合使用效果更佳,为临床应用提供了一定理论基础。

Objective It is to study the effects of volatile oil of Xihuang pill combined with human chorionic gonadotropins （HCG） on immune function in rats with breast cancer. Methods 84 rats which were successfully modelled breast cancer were randomly divided into three groups, each group had 28 rats. The rats in group A were given 0.9% physiological saline by layage and intraperitoneal injection, the rats in group B were given volatile oil of Xihuang pill by lavage with 0.9% physiological saline by intraperitoneal injection, the rats in group C was given volatile oil of Xihuang pill by lavage with HCG by intraperitoneal injection. The courses of treatment were 21 d, then the venous blood was extracted to detect the index of immue function and the rats were captured and then to measure the weight of tumor tissue in the three groups. Results The level of CD3^＋ , CD4^＋ , NK, IgG, IgM, IgA, IL-2, IL-4 and CD4^＋/CD8^＋ were lower than pretreatment （P 〈 0.05） , but the level of CDS, IL-12, and IL-6 were higher than pretreatment （P 〈 0.05） ; After treatment, the level of CD3^＋ , CD4^＋ , NK, IgG, IgM, IgA, IL-2, IL-4, and CD4^＋/CD8^＋ in group B and group C were higher than group A（P 〈 0.05） , but the level of CD8^＋ and IL-6, IL-12 were lower than group A （P 〈 0.05） ; After treatment, the level of CD3^＋ , CD4^＋ , NK, IgG, IgM, IgA, IL-2, IL-4, and CD4^＋/CD8^＋ in group C were higher than group B（P 〈0.05） , but the level of CD8^＋ and IL-6, IL-12 were lower than group B （P 〈 0.05 ）. The weight of tumor was lower in group B and group C than that in group A （P 〈 0.05 ） , and that in group B was lower than group C （P 〈 0.05 ） , the inhibiting rate of tumor in group C was higher than group B （P 〈 0.05 ）. Conclusion Volatile oil of Xihuang pill combined with HCG can improve the immune function and had anti-tumor activity in rats with breast cancer, and provide certain theoretical basis for clinical practice.