氯霉素对混养反硝化脱硫工艺运行效能研究

Effects of Chloramphenicol on Performance of Polyculture Denitrifying Sulfide Removal Process

采用UASB反应器考察氯霉素对反硝化脱硫工艺运行效能及微生物群落结构的影响。结果表明,氯霉素的投加对反硝化脱硫工艺影响不大。通过高通量测序技术分析微生物群落结构,发现氯霉素的存在会降低反应器中反硝化脱硫污泥微生物群落多样性。高氯霉素条件下,Thauera、Pseudomonas和Azoarcus是主要的异养反硝化微生物,Sulfurovum和Thiobacillus是主要的自养反硝化微生物,这些微生物使系统保持较好的硫氮同步脱除效果,此外,胞外聚合物尤其是胞外蛋白随氯霉素浓度增加而增加。

Using UASB reactor could explore the effects of chloramphenicol on the efficiency of denitrification and desulfurization and the structure of microbial community. Preliminary results showed that addition of chloramphenicol did not produce remarkable effect on the efficiency of denitrification and desulfurization. According to the high-throughput sequencing analysis method, existence of chloramphenicol will reduce the microbial community diversity of the desulfurization and denitrification sludge in the reactor. In the condition of high concentration of chloramphenicol,Thauera, Pseudomonas and Azoarcus were the major heterotrophic denitrifying microorganisms, Sulfurovum and Thiobacillus were the major autotrophic denitrifying microorganisms. Existence of these three microorganisms helped the system toj do a good job for simultaneous removal of sulfur and nitrogen. In addition,the amount of extracellular polymers,especially the extracellular proteins,increased with the rise of the concentration of chloramphenicol.