摘要
该文采用正交设计试验优化黄精多糖脱除蛋白的最佳条件。在单因素实验的基础上,以蛋白脱除时间、三氯乙酸浓度、蛋白脱除温度、蛋白质浓度为自变量,以黄精多糖中蛋白脱除率为考察指标,通过L9(34)正交试验得出黄精多糖蛋白脱除率的最佳工艺条件为:蛋白脱除时间40min、三氯乙酸浓度6%、蛋白脱除温度80℃、蛋白质浓度0.11mg/m L,在该条件下黄精多糖蛋白的脱除率为93.51%。
Optimization of the optimum conditions for deproteinization of polygonatum polysaccharide by orthogonal design.Orthogonal design was employed to evaluate the significance of four crucial variables including reaction time, concentration of trichloroacetic acid, deproteinization temperature, protein concentration, and to determine the effect of prime factors on the rate of deproteinization.The optimum conditions were reaction time 40min, concentration of tri- chloroacetic acid 6%, deproteinization temperature 80℃, and protein concentration 0.11mg/mL.Under these condi- tions, the rate of deproteinization was 93.51%.
出处
《安徽农学通报》
2017年第6期29-31,共3页
Anhui Agricultural Science Bulletin
基金
铜仁市科研项目(2016)80号
贵州省教育厅重点实验室项目[黔教合KY字(2011)005]
关键词
黄精多糖
三氯乙酸法
蛋白脱除
Polygonatum polysaccharide
Trichloroacetic acid
Deproteinization
