PTH基因多态性与骨质疏松性骨折的相关性研究

Association between PTH Gene Polymorphism and Osteoporotic Fracture

目的探讨甲状旁腺激素(parathyroid hormone,PTH)基因rs1459015位点多态性与北京地区汉族人群骨质疏松性骨折骨密度的关系。方法研究对象为北京地区无亲缘关系的汉族人群,其中骨质疏松性骨折患者(骨折组)127例,非骨质疏松性骨折患者(对照组)145例。采用SNaPshot法检验PTH基因多态性,双能X线吸收仪测定腰椎L2~4、股骨近端的neck、Ward’s三角、troch和total骨密度,计算两组等位基因频率、基因型频率,分析骨折组和对照组各部位骨密度差异以及骨折组PTH基因多态性与各部位骨密度的关系。结果骨折组A、G等位基因频率19.7%、80.3%,对照组A、G等位基因频率18.6%、81.4%,差异无统计学意义(P>0.05)。两组AA、AG、GG基因型频率分别为3.9%、31.5%、64.6%和3.4%、30.3%、66.2%,差异无统计学意义(P>0.05)。骨折组各部位骨密度均低于对照组,除neck骨密度差异外均有统计学意义(P<0.05)。骨折组中GG基因型腰椎骨密度均高于AA、AG基因型(P>0.05)。与AA、GG基因型相比,骨折组AG基因型髋部骨密度较高(P>0.05)。结论PTH基因多态性rs1459015位点与北京地区汉族人群骨质疏松性骨折患者骨密度不相关。

Objective To explore the relationship between the polymorphism of PTH gene rs1459015 and bone min-eral density（BMD）of osteoporotic fracture in Han nationality in Beijing.Methods The subjects included 127 osteopo-rotic fracture（fracture group）and 145 non-osteoporotic fracture（control group）.All subjects living in Beijing are Han Chinese without relationship.PTH gene polymorphisms were identified by SNaPshot.BMDs of the lumbar spine （L2~4 ） and proximal femur including neck,Ward·s,triangle troch and total hip were measured by dual energy X-ray absorpti-ometry.Allele frequency and genotype frequency were calculated.The BMD difference of two groups and the relationship of the PTH gene polymorphism with BMD of each location in fracture group were analyzed.Results The A and G allele frequency in two groups were 19.7%,80.3% and 18.6%,81.4%,without statistical significance（P〉0.05）.AA、AG、GG genotype frequencies of two populations were 3.9%,31.5%,64.6% and 3.4%,30.3%,66.2%,and this difference was not statistically significant（P〉0.05）.The BMDs of each location in fracture group were lower than those in control group,and the differences were statistically significant（P〈0.05）,not including the difference of BMD of neck.The BMDs of GG genotype were higher than those of AA and AG genotype in fracture group （P〉0.05）.Compared with AA and GG genotypes in fracture group,AG genotype had higher BMD of hip.Conclusion The PTH gene polymor-phism is not associated with BMD of osteoporotic fracture.