妊娠期糖尿病患者循环血液中β细胞营养因子水平变化的研究

Altering of circulating betatrophin in Pregnancy and Gestational Diabetes Mellitus

目的研究妊娠期糖尿病患者循环血液中β细胞营养因子(Betatrophin)水平的变化。方法选取2013年1年至2014年12月收治的38例妊娠期糖尿病(gestational diabetes mellitus,GDM)患者为研究对象,同期选择葡萄糖耐受正常(normal glucose tolerance,NGT)的孕妇38例及健康正常妇女(control)38例作为对照研究对象。酶联免疫吸附(ELISA)法检测血浆中betatrophin浓度,放射免疫法(RIA)进一步验证血浆中betatrophin浓度。结果经RIA验证的ELISA结果显示,GDM组血浆中betatrophin浓度(29.24±7.41ng/ml)显著高于NGT组(18.12±4.73ng/ml)和control组(0.47±0.15ng/ml),差异具有显著的统计学意义(P<0.001)。betatrophin与BMI和胰岛素抵抗无显著相关性。此外,Betatrophin浓度与孕期脂质参数包括TG和TC以及雌激素和孕激素呈显著相关。结论妊娠期糖尿病患者循环血液中betatrophin浓度显著高于NGT和正常孕妇,其可能作为妊娠期糖尿病患者血脂和血糖代谢异常的一个重要指标。

Objective To investigate the altering of circulating betatrophin in Pregnancy and Gestational Diabetes Mellitus. Methods Circulating betatrophin was quantified in 38 women with GDM and 38 pregnant body mass indexmatched women with normal glucose tolerance （NGT） as well as 38 healthy agematched non-pregnant women by enzyme-linked immunosorbent assay during January 2013 to December 2014. Additionally we performed radioimrnunassay （RIA） to confirm the results. Results Betatrophin concentrations measured by ELISA were significantly higher in GDM than in NGT and control （29.24±7. 41ng/ml vs 18. 12±4.73 ng/ml,P〈0. 001,29. 24±7. 41 ng/ml vs 0. 47±0.15 ng/ml,P〈0. 001） ,and also higher in NGT than control （18.12±4.73 ng/ml vs0.47±0.15 ng/ml,P〈0. 001） which was confirmed by RIA. Betatrophin did not correlate with BMI or insulin resistance in all women. Moreover it correlated significantly with lipid parameters including triglycerides and total cholesterol in pregnancy,as well as estrogen,progesterone. Conclusion Circulating betatrophin concentrations are dramatically increased in pregnancy and are significantly higher in GDM versus pregnant NGT, and betatrophin may represent a novel endocrine regulator of lipid alterations in pregnancy.