复合创伤继发颞下颌关节强直动物模型的建立

Establishment of animal model of temporomandibular joint ankylosis secondary to complex trauma

目的通过复合创伤模式,建立创伤性颞下颌关节强直动物模型,为进一步研究其机制提供实验载体。方法绵羊5只,除1只正常对照外,其余4只通过手术造成双侧髁突矢状骨折、关节窝损伤、关节盘部分切除的复合创伤。术后12周,通过螺旋CT检查、测量观察和组织学观察评价颞下颌关节的变化。结果 4只用于建模的绵羊双侧颞下颌关节均发生骨强直,CT检查发现髁突间隙内有钙化的骨痂形成,两侧关节面可见毛糙的新骨形成,伸入到关节间隙内。组织学观察可见关节间隙充满了丰富的新生软骨基质、软骨细胞和少量纤维组织。结论本研究成功建立了符合临床损伤方式及发病过程的创伤性颞下颌关节强直动物模型,为进一步研究其发病机制提供实验载体。

Objective To establish animal model of TMJ ankylosis secondary to complex trauma and to provide the experimental car- rier for the further research on its mechanism . Methods Five sheep were used in this study. Four were included in the operation group, while one was left as control. In the operation, bilateral TMJ osteotomies were performed on the four sheep to induce sagittal fractures of the mandibular condyle, the glenoid fossa was damaged and the lateral quarter of the disc was removed. At the end of the 12th week postoperatively, spiral CT scanning, gross observation and histological observation were used to evaluate the changes of the TMJ. Results Bilateral TMJ of the four sheep occurred bone ankylosis. CT scanning detected calcified callus formation in the joint space. Articular surfaces were roughened after the formation of new bone, which protruded into the joint space. Histological observation showed that joint space was filled with abundant new cartilaginous matrix, cartilage ceils, and a small amount of fibrous tissue. Conclusions This study successfully establishes the animal model of TMJ ankylosis resulting from complex trauma, which conforms to the clinical damage modes and disease process, and provides a stable and practical animal model for the further study of traumatic TMJ.