正交实验法优化发酵蜈蚣粉中总黄酮提取工艺研究

Study on Ultrasonic Extraction of Total Flavone from Ferment Centipede Powder with Orthogonal Experiment

目的:探究发酵蜈蚣粉中总黄酮的最佳提取工艺。方法:通过紫外可见分光光度法测定发酵蜈蚣粉中总黄酮含量,以总黄酮浸出量为考察指标,采用正交实验法,优化发酵蜈蚣粉中总黄酮的最佳提取工艺。结果:回流提取法提取总黄酮的最佳提取工艺为:提取温度70℃,提取时间1.5 h,料液比1:25。

Objective： To explore the best extraction process of total flavone from ferment centipede powder. Method： UV-vis was used to determine the content of total flavone and using orthogonal experiment to optimize it. Result： The best extracting process to extract total flavone with reflux extraction is under the power of ultrasound wave of 1： 25 g/m L,70 ℃ of the extraction temperation and 1. 5 h of the extraction time. Under these conditions,the content of flavone in extraction is 20. 311 mg/g.