四氧化三铁载银纳米粒子的原位制备及其抗菌应用

In-situ synthesis and antibacterial application of Fe_3O_4@Ag nanoparticles

采用高温裂解法制备四氧化三铁纳米粒子,并在其表面上通过还原乙酸银原位生长银纳米晶,制备特异形貌、均匀分散的四氧化三铁载银(Fe_3O_4@Ag)纳米粒子。利用透射电子显微镜(TEM)、傅里叶变换红外光谱(FT-IR)、X射线衍射仪(XRD)、振动样品磁强计(VSM)、紫外-可见分光光度计(UV-Vis)等表征技术对Fe_3O_4@Ag纳米粒子的结构和性能进行表征。通过分别研究实验制备的纳米颗粒对葡萄球菌和大肠杆菌的抗菌能力,分析Fe_3O_4@Ag纳米粒子的抗菌性能。结果表明,Fe_3O_4@Ag纳米粒子不仅具备四氧化三铁的室温超顺磁性,同时对葡萄球菌和大肠杆菌表现出优良的抗菌性能。利用如此方法构筑的多功能纳米材料——Fe_3O_4@Ag纳米粒子,将来在靶向抗菌、生物分离等领域具有重要的应用前景。

Nanocrystals acting as ideal candidates composed higher order of multifunctional materials on demands,such as Fe3O4nanoarticles and Ag nanocrystals.Constructed by functional＂building blocks＂,the multifunctional nanostructure materials had potential multiple applications,such as targeted antibacterial and biological separation.Referring to previous works,Fe3O4nanoparticles had been widely studied because of controllable morphology and excellent magnetic applications,and Ag nanoparticles performed excellent antibacterial properties and optical and catalysis applications.In special design and purpose,Ag loaded on magnetic nanocrystals or nanoparticles could be magnetic-operated using additional magnetic fields,and exhibited good antibacterial ability.To date,a series of strategies had been developed to build various nanostructure magnetic-antibacterial materials by assembling Ag nanocrystals with magnetic materials,such as self-assembly by H-bond,encapsulation with silicon and one-pot synthesis.Inthis work,Fe3O4@Ag nanoparticles were in-situ synthesized by Ag nanocrystals germinating on the surface of Fe3O4nanoparticle with oleic acid ligands.The process of preparing Fe3O4@Ag nanoparticles occurred through reducing silver acetate by oleylamine in hexane under 70 oC.For prudent study,the particles were characterized by TEM,XRD,FT-IR,VSM and UV-Vis spectroscopy.The TEM images and FT-IR spectroscopy claimed the characteristic morphology of well-dispersed Fe3O4@Ag nanoparticles with size about 20nm.X-ray diffraction patterns claimed that Fe3O4and Ag crystallization peaks existed as shown.Based on the VSM curve,Fe3O4@Ag nanoparticles shows superparamagnetic behavior under room temperature.UV-Vis spectroscopy claimed that the peaks according to Ag performed on the UV-Vis spectroscopy of Fe3O4@Ag nanoparticles.After phase-inversion of nanoparticles into deionized water was successful achieved,Fe3O4@Ag nanoparticles presented good antibacterial performance against staphylococcus and escherichia coli.