壮骨关节胶囊对SD大鼠骨性关节炎并骨质疏松症骨密度和雌激素受体表达的影响

Effect of Zhuangguguanjie capsule on bone mineral density and estrogen receptor expression in SD rats with osteoarthritis and osteoporosis

目的建立骨性关节炎+骨质疏松症SD大鼠模型,观察壮骨关节胶囊对SD大鼠骨性关节炎并骨质疏松症模型骨密度和雌激素受体表达的影响。方法取30只雌性未孕SD大鼠,随机分为对照组、骨性关节炎+骨质疏松症组(模型组)、骨性关节炎+骨质疏松症+壮骨关节胶囊组(治疗组),每组10只。模型组及治疗组采用去势法+改良Hulth法制作骨质疏松症模型+骨性关节炎模型,治疗组造模后12周给予壮骨关节胶囊连续8周。对照组大鼠仅切除卵巢周围少许脂肪组织和右膝少量滑膜。术后仅予等量生理盐水。8周后制作右侧股骨髁标本,光镜观察大鼠右侧股骨内髁关节软骨组织形态学变化,双能X射线观察大鼠右侧股骨骨密度变化,运用免疫组织化学方法检测雌性大鼠各组骨组织中雌激素受体(ER)的变化。结果光镜下观察模型组大鼠软骨层明显变薄,软骨细胞少,结构不规则且多有裂隙,可见到大量的同源软骨细胞,治疗组明显好转;模型组大鼠骨密度较对照组明显下降(P<0.01),治疗组经壮骨关节胶囊干预后骨密度上升(P<0.05)。免疫组化结果显示,按阳性细胞灰度比较,模型组ERα、ERβ表达量较对照组显著降低(P均<0.01),治疗组与模型组比较,ERα、ERβ表达量增加(P均<0.01)。结论壮骨关节胶囊对骨性关节炎合并骨质疏松症SD大鼠骨密度有上调作用,并提高成骨细胞ERα、ERβ的表达,但其有效作用机制尚需进一步研究探讨。

Obective To establish animal model of osteoarthritis （OA） plus osteoporosis （OP） and observe the effects of Zhuangguguanjie on bone mineral density and estrogen receptor （ER） in SD rats with OA and OP. Methods Thirty fe- male unpregnant SD rats were selected, and the rats were randomly divided into control group, model group and treatment group （n = 10 each）. The model of OA plus OP was established by ovariectomy and modified Huhh method in model group and treatment group ,and Zhuangguguanjie capsule was given for 8 weeks continuously 12 weeks after modeling in treatment group. In control group, a little fat tissues around the ovary and a small amount of right knee synovium were removed only, and equivalent distilled water was given after operation. Right femoral condyle specimen was prepared to observe morpholog- ic changes of articular cartilage tissues of femoral medial condyle under light microscope. Dual-energy X ray was used to observe the changes of right femur bone mineral density. Immunohistochemical methods was used to detect estrogen receptor （ER） expression in bone tissues. Results Findings under light microscope showed that in model group, cartilage layer thinned;chondrocytes decreased;structure was irregular and had many cracks;a large number of homologous chondrocytes can be seen. Aforementioned changes obviously improved in treatment group. X ray examination showed that compared with control group,bone mineral density decreased significantly in model group （P 〈 0.01）, while it increased in treatment group after drug intervention （P 〈 0.05 ）. hnmunohistochemical results showed that according to the comparison of positive cells grey value,the expression quantities of ERα and ERβ in model group decreased significantly compared with control group（ all P 〈0.01 ） , and the expression quantities of ERct and ERβ in treatment group increased significantly compared with model group（ all P 〈 0. 01 ）. Conclusion Zhuangguguanjie capsule can improve cartilage morphology, increase bone mineral density and expressions of ERa and ERβ in osteoblasts in SD rats with OA and OP,but its effective mechanism needs to be studied further.