广西1例HIV-1G亚型毒株全长基因序列的测定和分析

Near full-length genomic analysis of an HIV-1 G subtype strain identified in Guangxi Zhuang Autonomous Region

目的对广西1例HIV-1 G亚型毒株感染者体内病毒近似全长基因组进行序列测定并进行系统进化分析。方法对前期鉴定的1例HIV-1G亚型毒株感染者进行背景信息收集,并采集其外周血,提取病毒RNA,利用逆转录巢式-PCR技术分两段扩增并测定病毒近似全长基因序列。利用MEGA6软件对全长序列进行系统进化分析。结果获得了病毒近似全长基因组序列,长度为8847 bp。在系统进化树中,该毒株与G亚型参考株成簇,Bootstrap值为100%。系统溯源分析显示,该毒株与广西首次报道的G亚型JN106043毒株关系最近(Bootstrap值为100%,基因离散率为5%)。结论该毒株可能是由广西毒株传播所致,提示G亚型毒株在广西地区可能存在一定的局部流行,需进一步监测。该毒株序列的测定和分析对于广西地区HIV流行状况分析具有重要意义。

Objective To sequence and analyze the near full-length genome of an HIV-1 subtype G strain identified in Guangxi, China. Methods The demographic information of an individual infected with HIV-1 subtype G strain was investigated,whose peripherial blood was collected. Viral RNA in plasma was extracted. The near full-length genome of HIV was amplified in two halves using RT-nested-PCR. The PCR products were purified and sequenced. Phylogenetic analysis was made using MEGA6 software. Results A near full-length genome of 8847 bp was obtained. In the neighborjoining tree, the strain clustered with subtype G references ,as supported by the high Bootstrap value （100%）. The closest phylogenetie relationship was found between our strain and another subtype G strain （JN106043） previously identified in Guangxi, which was supported by the genetic distance （5%） and high Bootstrap value （100%）. Conclusion The strain identified in the study might have originated from subtype G strains in Guangxi, suggesting that subtype G has spread locally in Guangxi. Further surveillance of subtype G epidemic in Guangxi is necessary. The near full-length subtype G strain genome will provide information for the surveillance of HIV in Guangxi.