皮质抑素在人肾组织中及IgA肾病肾组织中的表达情况

Cortistatin expression in renal tissue of humans and IgA nephropathy patients

目的探讨皮质抑素(cortistatin,CST)在人肾组织中的表达情况及其在不同分级IgA肾病肾组织中的表达变化。方法收集10例肾癌切除术患者的癌旁正常肾组织,通过逆转录聚合酶链反应(RT-PCR)与Western印迹分别检测人肾组织中CST mRrNA与蛋白的表达情况,采用免疫组织化学(IHC)技术对CST于肾组织中的表达进行定位。根据Lee分级标准将IgA肾病分为3组:Ⅰ~Ⅱ级为A组、Ⅲ~Ⅳ级为B组、Ⅴ级为C组,每组分别收集10例IgA肾病患者的肾穿组织样本。采用IHC技术,检测正常肾组织及不同分级IgA肾病肾组织中CST的表达变化。对临床指标进行多重线性回归分析,评估其对IgA肾病肾组织内CST表达量的影响。结果 RT-PCR与Western印迹结果均表明肾组织内表达CST,IHC结果显示CST表达于肾小管上皮细胞。IgA肾病中,病理分级越高,肾小管中CST表达越多。多重线性回归分析显示,病理分级与肾组织中CST表达量相关(r=0.875,尸<0.01)。结论 CST可能参与IgA肾病病理损伤的炎症反应,发挥抗炎保护作用。

Objective To explore the condition of cortistatin（CST） expression in human renal tissue and the changes in the level of CST in IgA nephropathy（IgAN） of different degrees.Methods Ten tumor adjacent normal renal tissue samples were collected.The mRNA and protein expressions of CST in human renal tissue were detected by reverse transcription-polymerase chain reaction（RT-PCR） and Western blotting,respectively.Immunohistochemisty（IHC） was performed to locate the expression of CST in renal tissue.According to the grading system of Lee et al,IgAN was divided into three groups;grade Ⅰ-Ⅱ（group A）,grade Ⅲ-Ⅳ（group B）,and grade Ⅴ（group C）,and ten renal biopsy tissue samples were collected for each group.IHC was performed to detect the change in the level of CST in normal and IgAN renal tissue of different degrees.The effect of clinical indices on the level of CST in IgAN renal tissue was assessed by multiple linear regression analysis.Results RT-PCR and Western blotting showed that CST was expressed in renal tissue and IHC showed that CST was expressed on renal tubular epithelial cells.In IgAN,the higher the pathological grade was,the higher the expression of CST in renal tubules was.Multiple linear regression analysis showed that the pathological grade was associated with the expression of CST in renal tissue（r = 0.875,P〈 0.01）.Conclusion CST may participate in the inflammatory reaction of IgAN pathological injury and exert anti-inflammation effects.