离子液体在银杏叶黄酮分离纯化中的应用

The Application of Ionic Liquidin Extracting and Purifying Ginkgo Biloba Flavonoids

目的:本文就银杏叶中银杏黄酮的分离纯化进行研究,建立了离子液体/盐双水相体系分离纯化银杏黄酮的新方法,为银杏黄酮的开发应用提供了科学依据。方法:将银杏叶进行粗提取,测定其黄酮总量。应用溴化1-辛基-3-甲基咪唑[C_8mim]BF_4和溴化1-丁基-3-甲基咪唑[C_4mim]BF_4分别与(NH_4)_2SO_4组成的双水相体系纯化银杏黄酮,探讨离子液体与盐浓度、体系温度对银杏黄酮萃取效率的影响。结果:以槲皮素作对照品用高效液相定性测定,发现离子液体并没有改变银杏黄酮的化学结构。结论:离子液体双水相体系实现了对银杏黄酮粗提取物的分离、纯化。

Ginkgo biloba contains rich pharmacological active ingredients. Many Research showed that Ginkgo Biloba Flavonoids had very high value in medical study. This article was about the extraction and separation of Ginkgo Flavonids. A new method utilizing ionic liquid aqueous two-phase system（ATPS） was established. It provided a scientific basis for the development and application of Ginkgo Flavonids. The main contents and conclusion were as follows： Firstly, the total flavonoids were extracted from the leaves of Ginkgo biloba. Secondly study the purification of flavonoids of Ginkgo biloba bromide 1 octyl-3-methylimidazolium [C8mim]BF4 and bromide 1-butyl-3-methyl imidazolium [C4mim]BF4 respectively with（NH4）2SO4 composition of aqueous two-phase system, and discusses the effects of ionic liquid and salt concentration, system temperature on the extraction efficiency of Ginkgo flavone. Finally, using the high performance liquid chromatography analysis, the ionic liquid did not change the chemical structure of the ginkgo flavonoids, and realize the separation and purification of the crude extract of Ginkgo flavonoids by ionic liquid salt aqueous two-phase system.