高效液相色谱法测定污水厂中邻苯二甲酸酯类污染物

Determination of Phthalic Acid Esters in Wastewater Treatment Plant by High Performance Liquid Chromatography

建立了一种用高效液相色谱法(HPLC)测定污水中DMP、DEP、DBP和BBP 4种邻苯二甲酸酯的分析方法。HPLC分析条件:以C18色谱柱(4.6*250 mm,5μm)为分离柱,以乙腈-水(80∶20,v/v)为流动相,紫外检测器(检测波长238 nm),流速为1 mL/min。该方法操作简便、分析时间短,精密度与准确度良好,污水样加标回收率在88.59%~111.96%之间,相对标准偏差小于7.30%。污水厂水样测定结果表明,进水中4种PAEs污染较为严重,总浓度达到116.04μg/L,而出水总浓度降至6.29μg/L,污水处理工艺对4种邻苯二甲酸酯均具有较高的去除率。

To establish a high performance liquid chromatography（HPLC） method for the determination of four kinds of phthalic acid esters, including DMP, DEP, DBP and BBP. HPLC analysis conditions： C18 column（4.6＊250 mm,5 μm） as Separation column, acetonitrile-water（80：20） as mobile phase, UV detection（detecting wavelength was 238 nm）, and the flow rate was 1 ml/min. The method has the advantages of simple operation, short analysis time and good precision and accuracy for the recovery rate of sewage samples ranged from 88.59% to 111.96% and the relative standard deviations were less than 7.30%. The determination results of sewage samples showed that the pollution of four phthalates in influent was serious, with the total concentration of 116.04 μg/L, however the total effluent concentration felled to 6.29 μg/L because of the high removal rate of four phthalates by Sewage Treatment.