二甲亚砜提取——冷冻离心净化测定食品中苯并[a]芘及苯并[e]芘

Determination of benzo[a]pyrene and benzo[e]pyrene in food by extraction with dimethyl sulfoxide and purification with refrigerated centrifugation

建立以二甲基亚砜作为提取溶剂,冷冻离心提取净化食品中的苯并[a]芘和苯并[e]芘,高效液相色谱—荧光法检测的方法。替代传统的固相萃取小柱净化方式,能更快速、高效、准确检测食品中苯并[a]芘和苯并[e]芘的含量。2种目标物在0.5~1 000 ng/mL范围内呈现良好线性关系,在1~100 ng/g添加量下,回收率为89.0%~91.3%,相对标准偏差为1.19%~1.29%;仪器检出限为0.01 ng/mL,植物油脂方法检出限为0.05 ng/g,肉制品和动物油脂方法检出限为0.02 ng/g。该方法测定动植物油脂和肉制品中的苯并[a]芘和苯并[e]芘方便、快捷,适用于批量化检测。

Benzo[a]pyrene and benzo[e]pyrene in food was extracted and purified by refrigerated centrifugation with dimethyl sulfoxide as solvent and analyzed by HPLC-fluorescence detection method.This method can replace the traditional solid-phase extraction column purification method,which is faster,more efficient and accurate in the detection of benzo[a]pyrene and benzo[e]pyrene content.Both target object showed a good linear relationship within range of 0.5~1 000 ng/mL,when addition was 1~100 ng/g,the recovery rate was 89.0%~91.3%,and the relative standard deviation was1.19%~1.29%;the detection limit was 0.01 ng/mL,vegetable fat detection limit was 0.05 ng/g,meat and animal fat detection limit was 0.02 ng/g.This method is faster and more convenient in the detection of benzo[a]pyrene and benzo[e]pyrene in animal,vegetable fat and meat products,which is suitable for batch detection.