摘要
文章主要研究叶酸修饰硫掺杂二氧化钛(FA-S-TiO_2)纳米颗粒光催化灭活作用并初步探讨FA-S-TiO_2增强了TiO_2光催化灭活效率的作用机理。利用固相反应的方法制备了S-TiO_2,并利用表面修饰的方法制备了FA-S-TiO_2。通过紫外-可见光吸收光谱(UV-Vis),荧光光谱,傅里叶红外光谱(FTIR),透射电镜(TEM)等手段对样品进行了表征。利用Cell Counting Kit-8(CCK-8)法分别检测TiO_2、S-TiO_2、FA-S-TiO_2对HL60细胞的灭活效应,并采用活性氧检测等方法进行了分析研究。细胞实验结果表明,在暗室条件下,随着叶酸修饰S-TiO_2的比例增加,细胞的存活率随之减少;在光照条件下,细胞的存活率明显下降。其中FA-S-TiO_2样品1对HL60细胞的灭活效率最高,达到72%。其相应的活性氧含量也是最高。同时,通过荧光光谱推测FA-S-TiO_2提高HL60细胞对该纳米颗粒摄取效率,进而增强PDT灭活效果。
The photocatalytic inactivation efficiency of HL60 cells in vitro based on sulfur-doped titanium dioxide modi-fied folic acid （FA-S-TiO2） and its mechanism were researched in this paper. FA-S-TiO2 nanoparticles were synthesized by solid-phase reaction and surface modification method and were characterized by ultraviolet-visible absorption （UV- Vis）,fluorescence spectra, fourier transformation infrared spectrum （FTIR） , transmission electron microscope （TEM） and so on. The photocatalytic inactivation of these nanoparticles on HL60 cells was analyzed by CCK-8 assay and its mechanism was investigated using reactive oxygen species （ROS） detection. The results of cell experiment in the dark-room conditions showed that the viability of cells was reduced with the increment of FA. However, under the light expo-sure conditions, especially, FA-S-TiO2 sample 1 showed the highest cell inactivated efficiency of 72% , and the fluores-cence intensity of the reactive oxygen species was the strongest. Meanwhile, the fluorescence spectra indicated that FAS-TiO2 may improve the photocatalytic inactivation by improving uptake efficiency of nanoparticles.
出处
《激光生物学报》
CAS
2017年第1期30-36,49,共8页
Acta Laser Biology Sinica
基金
国家自然科学基金(61072029)
广州市科技计划资助项目(2014J4100049)
