中国荷斯坦奶牛Nramp1基因多态性与泌乳性状及产奶量的关联性分析

Genetic polymorphism Nramp1 gene and its associations with milk traits and milk yield in Chinese Holstein cattle

为寻找与乳房炎相关的分子标记,为荷斯坦牛的抗病育种提供理论基础,试验采用PCR-SSCP技术和测序的方法对宁夏农垦303头中国荷斯坦奶牛的Nramp1基因进行了遗传多态性分析,利用一般线性模型分析Nramp1基因c.200C>G突变对泌乳性状及产奶量影响。结果显示,Nramp1基因c.200C>G突变与乳脂率、日产奶量和体细胞评分值存在显著(P<0.05)或极显著(P<0.01)关联。因此,可以把该位点作为中国荷斯坦奶牛体细胞评分、测定日产奶量和日乳脂率的遗传标记进行进一步研究。

To search the molecular markers that are associated with mastitis and to provide a theoretical basis for the Holstein cattle breeding and disease resistance,the polymoprhisms of Nramp1 gene in 303 Holstein cattle in Ningxia were detected by the PCR-SSCP technique and the effect of Nramp1 gene c. 200 C G locus mutation on milk traits and milk yield was analyzed using a general linear model. The results showed that there was a significant relationship between Nramp1 gene c. 200 C 〉G locus mutation and milk fat,milk yield and somatic cell score at the probability level of 0. 05 or 0. 01. Therefore we can put the site as cattle somatic cell score,daily milk yield measurement and daily milk fat genetic markers in Chinese Holstein.