中山地区铜绿假单胞菌Ⅲ型分泌系统毒力基因表达差异的检测

Detection of type three secretion system virulence gene expression difference of pseudomonas aeruginosa in Zhongshan area

目的分析中山地区铜绿假单胞菌Ⅲ型分泌系统(TTSS)毒力基因表达及耐药情况,为临床抗感染治疗提供依据。方法对中山市人民医院2016年7-11月临床分离的铜绿假单胞菌随机选取76株,采用PCR法检测分离菌株exoU、exoS、exoT、exoY 4种毒力基因;采用Vitek2Compact全自动微生物鉴定仪测定细菌对抗菌药物的敏感性。结果 exoS、exoY检出率最高,分别为52.6%(40/76)、63.2%(48/76);主要为exoU(-)exoS(+)exoT(-)exoY(+)基因型,占59.26%;多重耐药及非多重耐药铜绿假单胞菌株4种毒力基因的阳性率无显著性差异;TTSS阳性组对13种药物的耐药率普遍低于TTSS阴性组,差异无统计学意义(P>0.05)。结论铜绿假单胞菌毒力基因携带存在一定的地域性差别;多重耐药及非多重耐药铜绿假单胞菌株毒力基因携带情况相似。

Objective To analyze the virulence genes expression and drug resistance situation of type three secretion system（TTSS）of pseudomonas aeruginosa in Zhongshan area to provide a basis for clinical anti-infection treatment.Methods Seventy-six clinically isolated strains of Pseudomonas aeruginosa were collected from the Zhongshan Municipal People′s Hospital from July to September 2016.Four virulence genes exoU,exoS,exoT and exoY were isolated from the strains by PCR.The Vitek2 Compact automatic microbiological identification instrument was used to detect the sensitivity of antibacterial drugs.The enumeration data were processed with chi-square test.Results The detection rates of gene exoS and exoY were highest,which were 52.6%（40/76）and63.2%（48/76）respectively;genotype exoU（-）exoS（＋）exoT（-）exoY（＋）were predominant and accounted for 59.26%.The positive rates of 4virulence genes had no statistical difference between the multiple resistant and non-multiple resistant strains of pseudomonas aeruginosa.The resistance rates in the TTSS positive group to 13 kinds of drugs were commonly lower than those in the TTSS negative group,the difference was statistically significant.Conclusion The geographic difference exists in virulence genes carrier of pseudomonas aeruginosa.The virulence genes carrying situation in both the multiple resistant and non-multiple resistant strains of pseudomonas aeruginosa is similar.