怀区熟地傅里叶变换中红外光谱指纹图谱研究

FTIR Fingerprint of Radix Rehmannia glutinosa Libosch Preparata in Fourier Transform

目的:建立怀区熟地的中红外指纹图谱.方法:利用傅立叶变换中红外光谱技术结合对比分析软件对不同炮制方法、炮制时间的10个熟地样品的红外光谱进行测定分析.结果:(1)建立了怀区熟地指纹图谱,得到13个共有特征峰;(2)不同熟地指纹谱相似度较好,说明怀区大多数熟地样品的组分、质量相近;(3)聚类分析结果表明,除9号、10号聚为一类,其他相同方法炮制的均聚为一类,可能是因为9号炮制时间为2012年,对熟地质量有影响.说明炮制方法、贮存时间对熟地品质有影响.结论:为鉴别不同炮制方法的熟地提供了一种简单、快捷、有效的手段,为地黄的深加工质量控制提供技术支撑.

Objective： To establish the FTIR fingerprintof Rehmannia glutinosa Libosch in Huai Zone . Methods： The FTIR fingerprint combined with contrast analysis software was used to analyze the infrared spectra of 10 cooked samples of dif-ferent processing methods and processing time. Results ： （1） FTIR fingerprint of Rehmannia glutinosa Libosch in Huai Zone were established, 13 of them were common characteristic peaks; （2） The similarities of fingerprints of different Rehmannia glutinosa Libosch were good？ which showed that the components and quality of most samples were similar. （3） The results of clustering analysis showed that, except for No. 9 and No. 10 were in one group, the other samples which were processed in the same methods were in one group, which was probably because the processing time of 9 was in 2012 and it affected the quality of Rehmannia glutinosa Libosch. The results showed that the processing method and storage time had an effect on the quality of Rehmannia glutinosa Libosch. Conclusion： It provided a simple, rapid and effective method for the identification of different processing methods of Rehmannia glutinosa Libosch, and provided a technical support for the deep processing quality control of Rehmannia glutinosa Libosch.