量子点-DNA电化学发光传感器的制备与表征

The Preparation and Characterization of Quantum Dots-DNA Electrochemiluminescence Sensor

为实现对特定基因序列的快速、灵敏检测,该文制备了一种基于碲化镉量子点的DNA电化学发光传感器并对测试条件进行了优化。电化学发光传感器是以金电极作基底,然后依次键合L-半胱氨酸和探针单链DNA。采用循环伏安法和电化学阻抗谱法对传感器的组装过程进行表征。电化学发光试剂碲化镉量子点标记的目标DNA与探针DNA杂交构成电化学发光传感器体系。结果表明,扫描时间6s,扫描电位-3.0^-1.5V,扫描速率0.1V/s时,电化学发光传感器体系的电化学发光效果最佳。

The CdTe quantum dots（QDs）-based DNA electrochemiluminescence（ECL）sensor was prepared for rapid and sensitive detection of the specific gene sequence.The proposed sensor used the gold electrode as the substrate,the L-cysteine and the probe of single-stranded DNA（ssDNA）were bonded successively.The assembly process of sensor was characterized by the cyclic voltammetry（CV）and electrochemical impedance spectroscopy（EIS）.The target ssDNA labeled by CdTe QDs and the probe ssDNA were hybridized to form an ECL sensing system.The result indicated that the optimum electrochemiluminescence performance was obtained at the scan time of 6s,scan potential of -3.0^-1.5V and scan rate of 0.1V/s.