维吾尔族女性乳腺癌与低分子量蛋白酶体蛋白表达缺失的关系

Loss of expression of low molecular-weight proteins in breast cancers of Uighur women

目的:探讨低分子量蛋白酶体(LMP)蛋白表达与乳腺癌的关系及其在维吾尔族和汉族女性中的表达差异。方法:收集维吾尔族和汉族女性乳腺纤维腺瘤及乳腺癌患者的石蜡包埋组织标本165例,应用免疫组织化学SP法检测LMP2、LMP7、LMP10蛋白的表达水平。结果:与乳腺纤维腺瘤组相比,LMP2和LMP7蛋白在乳腺癌组织中表达水平显著下降,表达完全缺失率明显上升,分别为67%和46.1%,差异均具有统计学意义(P<0.01);LMP10在乳腺纤维腺瘤和乳腺癌组中的表达缺失率分别为48%和56.5%,两组之间表达差异无统计学意义(P>0.05)。LMP2、LMP7和LMP10蛋白在维吾尔族女性乳腺癌患者中的表达率分别为43.1%、43.1%和47.7%,汉族分别为46%、42%和42%,不同民族女性患者之间表达的差异均无统计学意义(P均>0.05)。LMP2、LMP7和LMP10与乳腺癌淋巴结转移及远处转移相关,差异均有统计学意义(P均<0.05),同时LMP10表达降低与肿块大小有明显关系(P<0.01)。结论:LMP蛋白表达水平降低或缺失可能与乳腺癌的发生有关,其变化趋势在维吾尔族和汉族间有共性,可能反映乳腺癌发生的分子机制和特有的遗传背景。

OBJECTIVE：To compare the expression of low molecular-weight proteins （LMP） in breast cancers between Uighur and Han women.METHODS：We collected 165 cases of paraffin-embedded breast fibro adenoma or breast cancertissues from Uighur and Han women and analyzed the protein expression of LMP2,LMP7 and LMP10 by immunohistochemistry.RESULTS：The total loss rate of LMP2 and LMP7 was significantly increased in breast cancer （67%/46.1%）,the normal expression level was reduced with asignificant difference （P〈0.01）,the alternation trend of the loss rate of LMP10 was common to breast cancer and breast fibro adenoma （breast fibro adenoma 48%,breast cancer 56.5%）,and the difference between the two groups is not statistically significant （P〉0.05）. The alteration trend of the expression of LMP2,LMP7 and LMP10 was common to women from both Uighur and Han ethnic groups,in Uighur groups （43.1%,43.1%,47.7%）,in Han groups （46%,42%,42%） and the difference between two ethnic groups is not statistically significant （P〉0.05）. However,LMP2,LMP7 or LMP10 were significantly correlated with tumor lymph node invasion and distant metastasis （P〈0.05）,LMP10 was significantly correlated with tumor size （P〈0.01）.CONCLUSION：The loss of expression of LMP protein may be an important marker for breast cancer and was common to women from both Uighur and Han ethnic groups. This suggest that the loss may be a olecular mechanism for breast cancer and for both ethnic background.