逍遥散对肝郁气滞证视神经炎大鼠视网膜神经节细胞凋亡的影响

Effect on apoptosis of retinal ganglion cells in rats with optic nerve with Xiaoyao powder

目的:观察逍遥散对肝郁气滞证视神经炎大鼠视网膜神经节细胞(RGCs)凋亡的影响,为临床应用逍遥散治疗视神经炎提供实验室依据。方法:将60只大鼠按RAND函数随机分为正常组、肝郁气滞证视神经炎组(病证结合组)、逍遥散加肝郁气滞证视神经炎组(给药组),应用慢性应激方法建立肝郁气滞证动物模型,并采用EAE动物模型方法建立视神经炎动物模型,记录大鼠神经功能评分,光镜下观察视网膜形态学变化,利用上述指标评价造模成功。第14天、第28天时采用原位末端转移酶标记技术(TUNEL法)光镜下观察并记录3组大鼠视网膜神经节细胞(RCGs)凋亡数量,计算各组凋亡指数并比较。结果:在免疫后第14天,除正常组外其余两组视网膜均出现不同程度的病理改变,在免疫后28 d,病证结合组视网膜的病变的程度和范围较治疗后14 d时重;TUNEL法检测,14 d时,正常组大鼠视网膜未见凋亡,病症结合组及给药组可见一定数量的凋亡,两组与正常组比较均有统计学意义(P<0.05),28 d时,病证结合组及给药组大鼠仍可见一定数量的凋亡,但病证结合组大鼠RCGs较14 d增多,给药组大鼠较病证结合组大鼠凋亡数量减少,差异均有统计学意义(P<0.05)。结论:逍遥散对肝郁气滞证视神经炎有干预作用,一定程度上可以降低视网膜病变程度和降低视网膜神经节细胞的凋亡,其作用机制有待进一步探讨。

Objective：To observe the effect of Xiaoyao Powder on the apoptosis of retinal ganglion cells（RGCs）in rats with optic neuritis of liver-qi stagnation syndrome,and to provide experimental evidence for clinical application of Xiaoyao Powder in the treatment of optic neuritis. Methods：Sixty rats were randomly divided into three groups according to RAND function：normal group,optic stunting syndrome of liver-qi stagnation syndrome group,Xiaoyao Powder group and optic neuritis group of liver-qi stagnation syndrome group. The animal model of optic neuritis was established by EAE animal model. The neurological function score was recorded and the retinal morphological changes were observed under light microscope. The number of apoptotic cells in retinal ganglion cells（RCGs）of rats was observed and recorded by TUNEL method on the 14^（th）day and the 28^（th）day. The apoptotic index of each group was calculated and compared. Results：On the 14^（th） day after immunization,the pathological changes of the retina in the other two groups were discovered. At 28 days after immunization,the extent and area of the retinal lesions were higher than those of 14 days after treatment. TUNEL assay showed that there was no apoptosis in the retina of normal group at 14 days,and there was a certain number of apoptosis in the combination group and the treatment group. There was significant difference between the two groups and the normal group（P〈0.05）,the number of apoptotic cells was still decreased in the combination group and the control group,but the number of RCGs in the rats of the combination group was more than that in the control group,the difference was significant Statistical significance（P〈0.05）. Conclusion：Xiaoyao powder on the stagnation of stagnation of qi stagnation of the liver has the effect of intervention. To a certain extent,it can reduce the degree of retinal degeneration and reduce retinal ganglion cell apoptosis and its mechanism remains to be further explored.