远志多糖的纯化分离研究

Study on the isolation and purification of polysaccharides from Polygala tenuifolia

目的:对远志多糖进行纯化分离,观察其具有药用功效的单一多糖,为其药用功效进一步研究奠定基础。方法:采用水提醇沉法提取远志粗多糖,用酶法和Sevage法除蛋白,活性炭脱色,葡聚糖凝胶Sephadex G-100分离纯化,并对分离得到的多糖进行初步的结构分析。结果:分离纯化得到的远志单一多糖,紫外可见光260 nm、280 nm处有多糖无蛋白质和核酸的吸收峰,在红外光谱也有表现出多糖的特征官能团。结论:推测该多糖为β-吡喃糖,而非α-吡喃糖,并且不含蛋白质。

Objective：To study purification and isolation of polysaccharides from Polygala tenuifolia. Method：This experiment used water extracting and alcohol precipitation extraction of polysaccharides from Polygala tenuifolia,removing protein by enzymatic and Sevage,decoloration by activated carbon,and isolation and purification by Sephadex G-100,and prove that the extraction of polysaccharides from a single component. At the same time by using chromatography,UV spectroscopy,and infrared spectroscopy to a preliminary study on its structure. Results：It shows that the polysaccharide from Polygala tenuifolia without protein,infrared spectroscopy has shows some its own special features. Conclusion：Speculate that single polysaccharide is beta-tetrahydropyranyl sugar,rather not alpha-tetrahydropyranyl sugar and nonprotein-containing polysaccharides.