治愈的肺结核患者治疗前后血清蛋白双向电泳初步比较分析

Preliminary comparative analysis of serum proteins from cured pateints with pulmonary tuberculosis before and after treatment by two dimension electrophoresis

选取2013年于广州市胸科医院治疗的初治肺结核,并经1个常规抗结核治疗疗程（6个月）治愈的42例患者作为研究对象,采用双向凝胶电泳技术（two dimension electrophoresis,2-DE）对研究对象治疗前、后的混合血清全组蛋白进行电泳分离,以PDQuest 8.0软件根据蛋白质点数、等电点（isoelectric point,PI）、相对分子质量（relative molecular mass,Mr）等进行差异蛋白描述性分析.结果显示,研究对象治疗前、后差异蛋白点共有15个,治疗后血清相对应差异蛋白有12个下调,3个上调,Mr为16 200～76 800,PI为5.0～6.8.由此可见,治愈的肺结核患者治疗前后血清中存在特异性的差异蛋白,差异蛋白可能作为结核病的重要诊断标志物或者治疗靶点.

A total of 42 new tuberculosis （TB） cases seeking health care in Guangzhou Chest Hospital in 2013 were enrolled in this study.All the patients were cured after 6-month first-line anti-TB regimen.The two-dimensions electrophoresis （2-DE） was performed to separate the whole blood protein of patients at the initial and end of treatment,respectively.In addition,the isoelectric point （PI） and the relative molecular mass （Mr） were determined by PDQuest 8.0 software.Our results revealed that there were 15 protein spots exhibiting different profiles between these two periods.In addition,the expression levels of 12 protein spots of the post-treatment period were significantly higher than those of the pre-treatment period,while 3 protein spots were significantly decreased after anti-TB treatment.The Mr values of these proteins ranged from 16 200-76 800,and the PI values were from 5.0-6.8.On the basis of our findings,there were specific differential serum proteins in the cured tuberculosis patients before and after treatment.The differential proteins might serve as important diagnostic markers or therapeutic targets of TB.